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首页> 外文期刊>International Journal of Electrochemistry >Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA
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Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA

机译：循环伏安法探索抗癌药物盐酸阿霉素的电化学中间体，并通过模拟研究评价其与DNA的相互作用

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Electrochemical behavior of the anticancer drug doxorubicin hydrochloride was studied using cyclic voltammetry in aqueous medium using Hepes buffer (pH~7.4). At this pH, doxorubicin hydrochloride undergoes a reversible two-electron reduction withE1/2value −665±5 mV (versus Ag/AgCl, saturated KCl). Depending on scan rates, processes were either quasireversible (at low scan rates) or near perfect reversible (at high scan rates). This difference in behavior of doxorubicin hydrochloride with scan rate studied over the same potential range speaks of differences in electron transfer processes in doxorubicin hydrochloride. Attempt was made to identify and understand the species involved using simulation. The information obtained was used to study the interaction of doxorubicin hydrochloride with calf thymus DNA. Cathodic peak current gradually decreased as more calf thymus DNA was added. The decrease in cathodic peak current was used to estimate the interaction of the drug with calf thymus DNA. Nonlinear curve fit analysis was applied to evaluate the intrinsic binding constant and site size of interaction that was compared with previous results on doxorubicin hydrochloride-DNA interaction monitored by cyclic voltammetry or spectroscopic techniques.
机译：使用Hepes缓冲液（pH〜7.4）在水性介质中使用循环伏安法研究了抗癌药物盐酸阿霉素的电化学行为。在此pH下，阿霉素盐酸盐经历可逆的两电子还原，E1 / 2值为-665±5 mV（相对于Ag / AgCl，饱和KCl）。根据扫描速率，过程要么是准可逆的（低扫描率），要么接近完美的可逆性（高扫描率）。在相同电位范围内研究的扫描速率下，盐酸阿霉素的行为差异说明了盐酸阿霉素在电子转移过程中的差异。尝试通过模拟来识别和理解所涉及的物种。获得的信息用于研究盐酸阿霉素与小牛胸腺DNA的相互作用。随着添加更多的小牛胸腺DNA，阴极峰值电流逐渐降低。阴极峰值电流的减少用于估计药物与小牛胸腺DNA的相互作用。应用非线性曲线拟合分析来评估固有结合常数和相互作用的位点大小，并将其与以前用循环伏安法或光谱技术监测的盐酸阿霉素-DNA相互作用的结果进行比较。

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