...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Environmental Research and Public Health >Simultaneous Detection of Nine Key Bacterial Respiratory Pathogens Using Luminex xTAG ? Technology
【24h】

Simultaneous Detection of Nine Key Bacterial Respiratory Pathogens Using Luminex xTAG ? Technology

机译：使用Luminex xTAG同时检测9种关键细菌呼吸道病原体。技术

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Early diagnosis and treatment are crucial to the outcome of lower respiratory tract infections (LRTIs). In this study, we developed an assay combining multiplex PCR and Luminex technology (MPLT) for the detection of nine important respiratory bacterial pathogens, which frequently cause LRTIs. These were Streptococcus pneumoniae , Moraxella catarrhalis , Staphylococcus aureus , Streptococcus pyogenes , Haemophilus influenzae , Mycoplasma pneumoniae , Legionella spp., Pseudomonas aeruginosa , and Klebsiella pneumoniae . Through the hybridization reaction between two new synthesized multiplex PCR products and MagPlex-TAG Microspheres, we demonstrate that the detection limits for these nine pathogens were as low as 10 2 –10 3 CFU/mL. Furthermore, 86 clinical bronchoalveolar lavage fluid specimens were used to evaluate this method. Compared with the results of nine simplex real-time PCR reactions targeting these nine pathogens, this MPLT assay demonstrated a high diagnostic accuracy for Streptococcus pneumoniae (sensitivity, 87.5% and specificity, 100%). Furthermore, sensitivity and specificity for the other eight pathogens all attained 100% diagnostic accuracy. In addition, the consistency between MPLT and the nine real-time PCR reactions exceeded 98.8%. In conclusion, MPLT is a high-throughput, labor-saving and reliable method with high sensitivity and specificity for identifying nine respiratory pathogens responsible for LRTIs. Indeed, this assay may be a promising supplement to conventional methods used to diagnose LRTIs.
机译：早期诊断和治疗对下呼吸道感染（LRTIs）的结果至关重要。在这项研究中，我们开发了一种结合多重PCR和Luminex技术（MPLT）的检测方法，用于检测九种经常引起LRTI的重要呼吸道细菌病原体。这些是肺炎链球菌，卡他莫拉菌，金黄色葡萄球菌，化脓性链球菌，流感嗜血杆菌，肺炎支原体，军团菌属，铜绿假单胞菌和肺炎克雷伯菌。通过两个新合成的多重PCR产物与MagPlex-TAG微球之间的杂交反应，我们证明了这9种病原体的检出限低至10 2 –10 3 CFU / mL。此外，使用了86个临床支气管肺泡灌洗液样本来评估该方法。与针对这9种病原体的9种单工实时PCR反应的结果相比，该MPLT分析显示出对肺炎链球菌的高诊断准确性（灵敏度为87.5％，特异性为100％）。此外，对其他八种病原体的敏感性和特异性均达到100％的诊断准确性。另外，MPLT与9个实时PCR反应之间的一致性超过98.8％。总而言之，MPLT是一种高通量，省力且可靠的方法，具有高灵敏度和特异性，可识别负责LRTI的9种呼吸道病原体。实际上，该测定法可能是用于诊断LRTI的常规方法的有前途的补充。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号