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首页> 外文期刊>International Journal of Environmental Research and Public Health >Possible Impact of 190G > A CCR2 and Δ32 CCR5 Mutations on Decrease of the HBV Vaccine Immunogenicity—A Preliminary Report
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Possible Impact of 190G > A CCR2 and Δ32 CCR5 Mutations on Decrease of the HBV Vaccine Immunogenicity—A Preliminary Report

机译：190G> A CCR2和Δ32CCR5突变对HBV疫苗免疫原性降低的可能影响-初步报告

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Background : Chemokine genetic variations are involved in infectious diseases such as hepatitis B virus (HBV). Several allelic variants might, in theory, affect the outcome of vaccination. Objectives : This study was carried out to examine the associations of Δ32 CCR5 and 190G > A CCR2 polymorphisms with a response to a primary course of three HBV vaccinations. Methods : Between December 2014 and December 2016, patients from three randomly selected primary care clinics in the West Pomeranian region (Poland), 1 month after receiving the third dose of HBV vaccine, were enrolled. Enzyme-linked immunosorbent assay (ELISA) system version 3.0 was used to detect anti-HBs and anti-HBc totals. The identification of polymorphisms were performed by a polymerase chain reaction technique using a single primer extension assay. Genotype distributions of responders versus non-responders to HBV vaccination were compared on the basis of anti-HBs level. Results : In 149 patients (mean age 60 years) the mean anti-HBs level was 652.2 ± 425.9 mIU/mL (range: 0–1111.0 mIU/mL). There were 14.1% ( n = 21) non-responders to the HBV vaccine (anti-HBs < 10.0 mIU/mL). The wild type/Δ32 genotype of CCR5 gene was found in 18.1% participants, and 1.3% were Δ32/Δ32 homozygotes. The frequency of allele A of the CCR2 gene was 11.1%. Lower anti-HBs levels in Δ32/Δ32 homozygotes were observed (Me = 61 mIU/mL vs. Me = 660.2 mIU/mL; p = 0.048). As age was found to be a correlate to the anti-HBs titer ( r = ?0.218, p = 0.0075; 95% CI: ?0.366–?0.059)—an analysis of a co-variance was performed which found a statistically significant ( p = 0.04) difference in anti-HBs titres between Δ32/Δ32 homozygotes and other CCR5 genotypes. The association between anti-HBs titres and CCR2 genotypes was not statistically significant. Conclusions : Our study—which is a preliminary report that suggest this topic deserves further observation with larger sample sizes, different ethnicities, and other single nucleotide poly-morphisms (SNPs)—suggests the possible involvement of CCR5 polymorphism in impairing the immunologic response to HBV vaccination, predominantly in relation to the passage of time.
机译：背景：趋化因子遗传变异涉及诸如乙型肝炎病毒（HBV）的传染性疾病。从理论上讲，几种等位基因变异可能会影响疫苗接种的结果。目的：本研究旨在检查Δ32CCR5和190G> A CCR2多态性与对三种HBV疫苗接种的原发性反应的相关性。方法：在2014年12月至2016年12月之间，在第三次HBV疫苗接种后1个月，对来自西波美拉尼亚地区（波兰）的三个随机选择的初级保健诊所的患者进行了研究。 3.0版酶联免疫吸附测定（ELISA）系统用于检测抗HBs和抗HBc总量。多态性的鉴定通过使用单引物延伸测定的聚合酶链反应技术进行。根据抗HBs水平比较了HBV疫苗接种反应者和非反应者的基因型分布。结果：在149名患者（平均年龄60岁）中，平均抗HBs水平为652.2±425.9 mIU / mL（范围：0-1111.0 mIU / mL）。对HBV疫苗（抗HBs <10.0 mIU / mL）有14.1％（n = 21）的无反应。在18.1％的参与者中发现了CCR5基因的野生型/Δ32基因型，而1.3％是Δ32/Δ32纯合子。 CCR2基因的等位基因A的频率是11.1％。观察到在Δ32/Δ32纯合子中较低的抗HBs水平（Me = 61 mIU / mL对Me = 660.2 mIU / mL; p = 0.048）。由于年龄与抗HBs滴度相关（r =？0.218，p = 0.0075; 95％CI：？0.366–？0.059），因此对协方差进行了分析，发现其具有统计学意义（ p = 0.04）Δ32/Δ32纯合子与其他CCR5基因型之间的抗HBs滴度差异。抗HBs滴度与CCR2基因型之间的相关性无统计学意义。结论：我们的研究是一项初步报告，表明该主题在较大样本量，不同种族和其他单核苷酸多态性（SNP）下值得进一步观察，建议CCR5多态性可能参与削弱对HBV的免疫反应疫苗接种，主要与时间的流逝有关。

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