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首页> 外文期刊>International Journal of Environmental Research and Public Health >Correlation of Internal Exposure Levels of Polycyclic Aromatic Hydrocarbons to Methylation of Imprinting Genes of Sperm DNA
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Correlation of Internal Exposure Levels of Polycyclic Aromatic Hydrocarbons to Methylation of Imprinting Genes of Sperm DNA

机译：多环芳烃内部暴露水平与精子DNA印迹基因甲基化的相关性

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摘要

Human exposure to polycyclic aromatic hydrocarbons (PAHs) results in adverse health implications. However, the specific impact of paternal preconception PAHs exposure has not been fully studied. In this study, a total of 219 men aged 24–53 were recruited and an investigation was conducted using a questionnaire requesting information about age, occupation, education, family history, lifestyle, and dietary preferences. Urine and semen samples were examined for the levels of the hydroxyl metabolites of PAHs (OH-PAHs) using ultra-high-performance liquid chromatography–tandem mass spectrometry and sperm DNA methylation by pyrosequencing. The results from the correlation analysis using seven OH-PAHs and the average methylation levels of the imprinting genes H19 , PEG3 , and MEG3 indicated that 1-OHPH is positively correlated with H19 / PEG3 methylation levels. We further examined the correlation between each OH-PAH and the methylation levels at the individual CpGs. The results showed 1-OHPH is specifically correlated with CpG4 and CpG6 of the imprinted gene H19 , CpG1 and CpG2 of PEG3, and CpG2 of MEG3 ; whereas 1-OHP is positively correlated with PEG3 at CpG1. Multivariate regression model analysis confirmed that 1-OHPH and 1-OHP are independent risk factors for the methylation of H19 . These data show that sperm DNA imprinting genes are sensitive to adverse environmental perturbations.
机译：人类暴露于多环芳烃（PAH）会对健康产生不利影响。但是，尚未充分研究父本受孕前PAHs暴露的具体影响。在这项研究中，共招募了219名年龄在24-53岁之间的男性，并使用问卷进行了调查，该问卷要求提供有关年龄，职业，教育程度，家族史，生活方式和饮食偏好的信息。使用超高效液相色谱-串联质谱法和焦磷酸测序法检测精子DNA甲基化，检测尿液和精液中PAHs（OH-PAHs）的羟基代谢产物水平。使用七个OH-PAHs和印迹基因H19，PEG3和MEG3的平均甲基化水平的相关分析结果表明，1-OHPH与H19 / PEG3甲基化水平呈正相关。我们进一步检查了每个OH-PAH与各个CpGs甲基化水平之间的相关性。结果表明，1-OHPH与印迹基因H19的CpG4和CpG6，PEG3的CpG1和CpG2以及MEG3的CpG2具体相关;而1-OHP与CpG1处的PEG3正相关。多元回归模型分析证实1-OHPH和1-OHP是H19甲基化的独立危险因素。这些数据表明，精子DNA印迹基因对不利的环境扰动敏感。

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