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首页> 外文期刊>International Journal of Environmental Research and Public Health >Assessment and Molecular Characterization of Human Intestinal Parasites in Bivalves from Orchard Beach, NY, USA
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Assessment and Molecular Characterization of Human Intestinal Parasites in Bivalves from Orchard Beach, NY, USA

机译：美国果园海滩双壳类中人体肠道寄生虫的评估和分子表征

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摘要

Bivalves have been shown to be carriers of the human intestinal parasites Cryptosporidium parvum and Toxoplasma gondii. The goal of this study is to determine the prevalence of protozoan parasites in mollusks of New York City using a polymerase chain reaction (PCR)-based assay. Four species of mollusks, Mya arenaria, Geukensia demissa, Crassostrea virginica, and Mytilis edulis, were collected from Orchard Beach, NY in the fall of 2014, totaling 159 specimens. Each individual mollusk was dissected to harvest the digestive gland, the mantle, the gills, the foot and the siphon. The tissues were assayed for the presence of Cryptosporidium parvum, Giardia lamblia, and Toxoplasma gondii DNA by using primers that target parasite-specific genes. C. parvum was found at a prevalence of 50%, 11.3%, and 1%, respectively, in Mya arenaria, G. demissa, and Mytilis edulis. C. parvum DNA was detected in all the tissues of these bivalve species, except the gills. Furthermore, G. lamblia was detected in Mya arenaria, G. demissa, Crassostrea virginica and Mytilis edulis at a prevalence of 37.5%, 4.5%, 60%, and 20.6%, respectively, while T. gondii DNA was not detected.
机译：双壳类已被证明是人类肠道寄生虫小隐孢子虫和弓形虫的携带者。这项研究的目的是确定基于聚合酶链反应（PCR）的纽约市软体动物中原生动物寄生虫的患病率。 2014年秋季，从纽约的乌节海滩收集了四种软体动物，分别为：Mya arenaria，Geukensia demissa，Crassostrea virginica和Mytilis edulis，共159个标本。解剖每个软体动物以收获消化腺，地幔，腮，足和虹吸管。通过使用靶向寄生虫特异性基因的引物，分析组织中是否存在小隐孢子虫，贾第鞭毛虫和弓形虫DNA。斑纹小球藻，米氏假丝酵母和可食的Mytilis紫丁香的患病率分别为50％，11.3％和1％。在这些双壳类物种的所有组织中（except除外）都检测到了小球藻DNA。此外，在Mena arenaria，G。demissa，Crassostrea virginica和Mytilis edulis中检出了G. lamblia，检出率分别为37.5％，4.5％，60％和20.6％，而未检出弓形虫DNA。

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