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Biodegradation of the High Explosive Hexanitrohexaazaiso-wurtzitane (CL-20)

机译:高爆炸性六硝基六氮杂异纤锌矿烷(CL-20)的生物降解

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The aerobic biodegradability of the high explosive CL-20 by activated sludge and the white rot fungus Phanerochaete chrysosporium has been investigated. Although activated sludge is not effective in degrading CL-20 directly, it can mineralize the alkaline hydrolysis products. Phanerochaete chrysosporium degrades CL-20 in the presence of supplementary carbon and nitrogen sources. Biodegradation studies were conducted using various nutrient media under diverse conditions. Variables included the CL-20 concentration; levels of carbon (as glycerol) and ammonium sulfate and yeast extract as sources of nitrogen. Cultures that received CL-20 at the time of inoculation transformed CL-20 completely under all nutrient conditions studied. When CL-20 was added to pre-grown cultures, degradation was limited. The extent of mineralization was monitored by the 14CO2 time evolution; up to 51% mineralization was achieved when the fungus was incubated with [14C]-CL-20. The kinetics of CL-20 biodegradation by Phanerochaete chrysosporium follows the logistic kinetic growth model.
机译:研究了活性污泥和白腐真菌Phanerochaete chrysosporium对高炸药CL-20的好氧生物降解性。尽管活性污泥不能直接降解CL-20,但可以使碱性水解产物矿化。在补充碳源和氮源的存在下,Phanerochaete chrysosporium会降解CL-20。在各种条件下使用各种营养培养基进行了生物降解研究。变量包括CL-20浓度;碳(以甘油为单位),硫酸铵和酵母提取物作为氮源的水平。在研究的所有营养条件下,接种时接受CL-20的培养物都将CL-20完全转化。当将CL-20添加到预生长的培养物中时,降解受到限制。矿化程度由14CO2时间演变来监测。当真菌与[14C] -CL-20孵育时,可实现高达51%的矿化。 Phanerochaete chrysosporium对CL-20生物降解的动力学遵循逻辑动力学生长模型。

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