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首页> 外文期刊>International Journal of Environmental Research and Public Health >Trichloroethylene and Its Oxidative Metabolites Enhance the Activated State and Th1 Cytokine Gene Expression inJurkat Cells
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Trichloroethylene and Its Oxidative Metabolites Enhance the Activated State and Th1 Cytokine Gene Expression inJurkat Cells

机译：三氯乙烯及其氧化代谢产物增强Jurkat细胞的激活状态和Th1细胞因子基因的表达

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Trichloroethylene (TCE) is an occupational and ubiquitous environmental contaminant, and TCE exposure will increase the risk of autoimmune diseases and allergic diseases. T cells play an important role in the pathogenesis of TCE-related immune disorders, but the effect of TCE and its oxidative metabolites, trichloroacetic acid (TCA) and dichloroacetic acid (DCA), on the activation of human T cells is still unknown. In this study, Jurkat cells were pre-treated with TCE, TCA and DCA overnight and then stimulated with phorbol 12-myristate 13-acetate and ionomycin for another 4, 8 and 24 hours. IL-2 secretion was detected by ELISA; the expressions of CD25 and CD69 were tested by flow cytometry; and IFN-γ and IL-2 mRNA expression levels were investigated by real-time PCR. The results showed that TCE and its oxidative metabolites, TCA and DCA, significantly enhanced IL-2 releasing and the expression of T cell activation markers, CD25 and CD69. Consistent with this result, these compounds markedly up-regulated the expression levels of IFN-γ and IL-2 mRNA. Collectively, these findings suggest that TCE and its metabolites, TCA and DCA, might enhance the activation of T cells and disrupt various activities of peripheral T cells.
机译：三氯乙烯（TCE）是一种职业和普遍存在的环境污染物，接触TCE会增加自身免疫性疾病和过敏性疾病的风险。 T细胞在TCE相关的免疫疾病的发病机理中起着重要作用，但是TCE及其氧化代谢产物三氯乙酸（TCA）和二氯乙酸（DCA）对人类T细胞活化的作用仍是未知的。在这项研究中，Jurkat细胞用TCE，TCA和DCA预处理过夜，然后再用佛波醇12-肉豆蔻酸酯13-乙酸酯和离子霉素刺激另外4、8和24小时。通过ELISA检测IL-2分泌;流式细胞术检测CD25和CD69的表达。实时荧光定量PCR检测IFN-γ和IL-2 mRNA表达水平。结果表明，TCE及其氧化代谢产物TCA和DCA显着增强了IL-2的释放以及T细胞活化标志物CD25和CD69的表达。与该结果一致，这些化合物显着上调了IFN-γ和IL-2 mRNA的表达水平。总的来说，这些发现表明TCE及其代谢产物TCA和DCA可能增强T细胞的活化并破坏外周T细胞的各种活性。

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