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首页> 外文期刊>International Journal of Environmental Research and Public Health >Effect of Light Irradiation and Sex Hormones on Jurkat T Cells: 17β-Estradiol but Not Testosterone Enhances UVA-Induced Cytotoxicity in Jurkat Lymphocytes
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Effect of Light Irradiation and Sex Hormones on Jurkat T Cells: 17β-Estradiol but Not Testosterone Enhances UVA-Induced Cytotoxicity in Jurkat Lymphocytes

机译：光照和性激素对Jurkat T细胞的影响：17β-雌二醇而非睾丸激素增强UVA诱导的Jurkat淋巴细胞的细胞毒性

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In Eastern cultures, such as India, it is traditionally recommended that women but not men cover their heads while working in the scorching sun. The purpose of this pilot study was to determine whether there was any scientific basis for this cultural tradition. We examined the differential cytotoxic effects of ultraviolet A light (UVA) on an established T cell line treated with female and male sex hormones. CD4+ Jurkat T cells were plated in 96 well plates at 2 × 106 cells/ml and treated with 17β-estradiol (EST) or testosterone (TE). These cells were irradiated by UVA light with an irradiance of 170 J/cm2 for 15min at a distance of 6 cm from the surface of the 96-well plate. Controls included cells not treated with hormones or UVA. The effects of EST and TE were investigated between 1 and 20 ng/mL. Cytotoxicity by fluorescein-diacetate staining and COMET assay generating single strand DNA cleavage, tail length and tail moment measurements were examined. The effect of estrogen (5ng/mL) on apoptosis and its mediators was further studied using DNA laddering and western blotting for bcl-2 and p53. We found that EST alone, without UVA, enhanced Jurkat T cell survival. However, EST exhibited a dose-related cytotoxicity in the presence of UVA; up to 28% at 20 ng/ml. TE did not alter UVA-induced cytotoxicity. Since TE did not alter cell viability in the presence of UVA further damaging studies were not performed. COMET assay demonstrated the harmful effects of EST in the presence of UVA while EST without UVA had no significant effect on the nuclear damage. Apoptosis was not present as indicated by the absence of DNA laddering on agarose gel electrophoresis at 5ng/ml EST or TE ± UVA. Western blot showed that estrogen down regulated bcl-2 independently of UVA radiation while p53 was down regulated in the presence of UVA treatment. EST and TE have differential effects on UVA-induced cytotoxicity in Jurkat T-lymphocyte which suggested that women may be more susceptible to the harmful effects of solar irradiation than men.
机译：在印度等东方文化中，传统上建议女性在烈日下工作时，男人要掩盖自己的头，而不是男人。这项初步研究的目的是确定这种文化传统是否有任何科学依据。我们检查了紫外线A光（UVA）对已建立的用女性和男性性激素治疗的T细胞系的不同细胞毒性作用。将CD4 + Jurkat T细胞以2×10 6 细胞/ ml接种在96孔板中，并用17β-雌二醇（EST）或睾丸激素（TE）处理。在距96孔板表面6 cm处，以170 J / cm 2 的UVA光照射这些细胞15分钟。对照包括未用激素或UVA处理的细胞。在1至20 ng / mL之间研究了EST和TE的作用。通过荧光素二乙酸盐染色和产生单链DNA裂解的COMET测定法检测细胞毒性，检测尾巴长度和尾矩。使用DNA阶梯电泳和蛋白质印迹法对bcl-2和p53进一步研究了雌激素（5ng / mL）对细胞凋亡及其介质的影响。我们发现单独的EST，没有UVA，可以增强Jurkat T细胞的存活率。然而，在UVA存在下，EST表现出剂量相关的细胞毒性。 20 ng / ml时高达28％。 TE不会改变UVA诱导的细胞毒性。由于TE在UVA存在下不会改变细胞活力，因此未进行进一步的破坏研究。 COMET分析证明存在UVA时EST的有害作用，而不含UVA的EST对核损伤无明显影响。如在5ng / ml EST或TE±UVA的琼脂糖凝胶电泳上没有DNA梯形显示，则不存在凋亡。蛋白质印迹显示，雌激素下调bcl-2不受UVA辐射的影响，而p53在进行UVA处理时下调。 EST和TE对UVA诱导的Jurkat T淋巴细胞的细胞毒性具有不同的影响，这表明女性可能比男性更容易受到太阳辐射的有害影响。

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