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首页> 外文期刊>International Journal of Environmental Research and Public Health >Sulforaphane Cannot Protect Human Fibroblasts From Repeated, Short and Sublethal Treatments with Hydrogen Peroxide
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Sulforaphane Cannot Protect Human Fibroblasts From Repeated, Short and Sublethal Treatments with Hydrogen Peroxide

机译：萝卜硫素不能保护人类成纤维细胞免受过氧化氢的反复，短暂和亚致死性治疗。

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A delicate balance of reactive oxygen species (ROS) exists inside the cell: when the mechanisms that control the level of ROS fail, the cell is in an oxidative stress state, a condition that can accelerate aging processes. To contrast the pro-aging effect of ROS, the supplementation of antioxidants has been recently proposed. Sulforaphane (SFN) is an isothiocyanate isolated from Brassica plants that has been shown to modulate many critical factors inside the cells helping to counteract aging processes. In the present work, we exposed human dermal fibroblast to short, sublethal and repeated treatments with hydrogen peroxide for eight days, without or in combination with low concentration of SFN. Hydrogen peroxide treatments did not affect the oxidative status of the cells, without any significant change of the intracellular ROS levels or the number of mitochondria or thiols in total proteins. However, our regime promoted cell cycle progression and cell viability, increased the anti-apoptotic factor survivin and increased DNA damage, measured as number of foci positive for γ -H2AX. On the other hand, the treatment with SFN alone seemed to exert a protective effect, increasing the level of p53, which can block the expansion of possible DNA damaged cells. However, continued exposure to SFN at this concentration could not protect the cells from stress induced by hydrogen peroxide.
机译：细胞内部存在活性氧（ROS）的微妙平衡：当控制ROS水平的机制失效时，细胞处于氧化应激状态，这种状态会加速衰老过程。为了对比ROS的延缓衰老的作用，最近已经提出了抗氧化剂的补充。萝卜硫素（SFN）是一种从芸苔属植物中分离出来的异硫氰酸盐，已被证明可以调节细胞内的许多关键因子，从而有助于对抗衰老过程。在目前的工作中，我们将人皮肤成纤维细胞暴露于短时间，亚致死和反复用过氧化氢处理八天，而无或与低浓度的SFN结合使用。过氧化氢处理不会影响细胞的氧化状态，总蛋白中细胞内ROS水平或线粒体或硫醇的数量没有任何显着变化。然而，我们的方案促进了细胞周期进程和细胞活力，增加了抗凋亡因子survivin并增加了DNA损伤（以γ-H2AX阳性灶的数量衡量）。另一方面，单用SFN的治疗似乎发挥了保护作用，增加了p53的水平，可以阻断可能的DNA损伤细胞的扩增。但是，持续暴露于此浓度的SFN不能保护细胞免受过氧化氢诱导的压力。

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