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首页> 外文期刊>International Journal of Environmental Research and Public Health >Quantitative RT-PCR Detection of Hepatitis A Virus, Rotaviruses and Enteroviruses in the Buffalo River and Source Water Dams in the Eastern Cape Province of South Africa
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Quantitative RT-PCR Detection of Hepatitis A Virus, Rotaviruses and Enteroviruses in the Buffalo River and Source Water Dams in the Eastern Cape Province of South Africa

机译：定量RT-PCR检测南非东开普省布法罗河和水源水坝中的甲型肝炎病毒，轮状病毒和肠病毒

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Human enteric viruses (HEntVs) are a major cause of water-related diseases. The prevalence of hepatitis A virus (HAV), rotaviruses (RoV) and enteroviruses (EnV) in Buffalo River waters was assessed quantitatively over a period of 12 months (August 2010 to July 2011). Seventy-two samples were collected from six sites, including three dams, and concentrated using the adsorption-elution method. Viral RNA was extracted using a commercial kit, and the viruses were quantified by real-time quantitative reverse transcriptase PCR (RT-qPCR). Two or more viruses were detected in 12.5% of the samples. HAV was detected in 43.1% of the samples and in significantly (p 0.05) varying concentrations of 1.5 × 101–1.9 × 105 genome copies/L compared to RoV and EnV, while RoVs were detected in 13.9% of samples, with concentrations ranging from 2.5 × 101–2.1 × 103 genome copies/L, and EnV were detected in 9.7% of the samples, with concentrations ranging from 1.3 × 101–8.6 × 101 genome copies/L. Only HAV was detected at all the sites, with the Bridle Drift Dam recording significantly higher (p 0.05) concentrations. The presence of enteric viruses in Buffalo River may constitute public health risks and the incidence of HAV at all the sites could reflect both the epidemiological status of hepatitis A and HAV persistence in the water environments.
机译：人类肠道病毒（HEntVs）是与水有关的疾病的主要原因。在12个月内（2010年8月至2011年7月），对布法罗河水域中的甲型肝炎病毒（HAV），轮状病毒（RoV）和肠病毒（EnV）的患病率进行了定量评估。从六个地点（包括三个水坝）收集了72个样品，并使用吸附-洗脱方法进行了浓缩。使用商业试剂盒提取病毒RNA，并通过实时定量逆转录酶PCR（RT-qPCR）定量病毒。在12.5％的样本中检测到两种或更多种病毒。与RoV相比，在43.1％的样品中检测到HAV，并且以1.5×10 1 –1.9×10 5 基因组拷贝/ L的显着浓度（p <0.05）检测到和EnV，而在13.9％的样本中检测到RoV，浓度范围为2.5×10 1 –2.1×10 3 基因组拷贝/ L，而EnV在样本的9.7％，浓度范围为1.3×10 1 –8.6×10 1 基因组拷贝/ L。在所有地点仅检测到HAV，而dle流坝的浓度则明显更高（p <0.05）。布法罗河中存在肠道病毒可能构成公共卫生风险，并且所有地点的甲肝病毒感染率都可能反映甲型肝炎的流行病学状况和乙肝病毒在水环境中的持久性。

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