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首页> 外文期刊>International Journal of Environmental Research and Public Health >Evaluation of Three Swabbing Devices for Detection of Listeria monocytogenes on Different Types of Food Contact Surfaces
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Evaluation of Three Swabbing Devices for Detection of Listeria monocytogenes on Different Types of Food Contact Surfaces

机译：三种擦拭设备在不同类型食品接触表面上检测单核细胞增生李斯特菌的评估

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Listeria monocytogenes can adhere to different types of food contact surfaces within a food processing environment. Therefore, environmental sampling devices should be capable of detecting unacceptable contamination. In this study, a sponge-stick, foam spatula and an environmental swab were evaluated on their ability to detect low concentrations of L. monocytogenes on different types of food contact surfaces. A cocktail of four L. monocytogenes serotypes was inoculated with a concentration of 100 CFU/250 cm2 onto stainless steel (SS), high density polyethylene (HDPE) and rubber surfaces in a 250 cm2 area. Immediately after inoculation and after 1 h exposure, the surfaces were swabbed with the different swabbing devices. The results of the study show only minor differences in the ability of the swabbing devices to detect L. monocytogenes. All devices were capable to detect the contamination immediately after inoculation. However, when the surfaces were allowed to air-dry for 1 h, L. monocytogenes was undetected in 11.1% of the samples (n = 27) with the sponge stick, in 7.4% of the samples (n = 27) with the foam spatula and in 3.7% of the samples (n = 27) with the environmental swab, especially on SS surfaces. The detection ability of the different devices for L. monocytogenes can be concluded to be rather high on different types of food contact surfaces.
机译：单核细胞增生李斯特菌可以粘附在食品加工环境中的不同类型的食品接触表面上。因此，环境采样设备应能够检测到不可接受的污染。在这项研究中，评估了海绵棒，泡沫刮铲和环境拭子在不同类型的食物接触表面上检测低浓度单核细胞增生李斯特氏菌的能力。将浓度为100 CFU / 250 cm2的四种单核细胞增生李斯特菌血清型混合物接种到250 cm2面积的不锈钢（SS），高密度聚乙烯（HDPE）和橡胶表面上。接种后和暴露1小时后，立即用不同的擦拭设备擦拭表面。研究结果表明，拭子设备检测单核细胞增生李斯特菌的能力仅有微小差异。接种后，所有设备均能够立即检测到污染。但是，将其表面风干1小时后，用海绵棒在11.1％的样品（n = 27）中检测不到单核细胞增生李斯特氏菌，在使用泡沫的样品中有7.4％的样品（n = 27）未检出单核细胞增生李斯特氏菌。刮刀和3.7％的样本（n = 27）中带有环境拭子，尤其是在SS表面。可以得出结论，在不同类型的食物接触表面上，单核细胞增生李斯特氏菌的不同设备的检测能力相当高。

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