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首页> 外文期刊>International Journal of Environmental Research and Public Health >Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle’s Markets in Ouagadougou, Burkina Faso
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Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle’s Markets in Ouagadougou, Burkina Faso

机译：从布基纳法索瓦加杜古牛市场的有机废物（牛粪，粪便和浆液）中分离的腹泻性大肠杆菌的特征

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Cattle farming can promote diarrheal disease transmission through waste, effluents or cattle fecal matter. The study aims to characterize the diarrheagenic Escherichia coli (DEC) isolated from cattle feces, manure in the composting process and slurry, collected from four cattle markets in Ouagadougou. A total of 585 samples (340 cattle feces, 200 slurries and 45 manures in the composting process) were collected from the four cattle markets between May 2015 and May 2016. A multiplex Polymerase Chain Reaction (PCR), namely 16-plex PCR, was used to screen simultaneously the virulence genes specific for shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and enteroaggregative E. coli (EAEC). DEC was detected in 10.76% of samples. ETEC was the most prevalent (9.91%). STEC and EAEC have been observed with the same rate (0.51%). ETEC were detected in 12.64% of cattle feces, in 6.66% of manure in the composting process and in 5% of slurry. STEC were detected in 0.58% of cattle feces and in 2.22% of manure in the composting process. EAEC was detected only in 1% of slurry and in 2.22% of manure in the composting process. ETEC strains were identified based on estIa gene and/or estIb gene and/or elt gene amplification. Of the 58 ETEC, 10.34% contained astA , 17.24% contained elt , 3.44% contained estIa and 79.31% contained estIb . The two positive EAEC strains contained only the aggR gene, and the third was positive only for the pic gene. The results show that effluent from cattle markets could contribute to the spreading of DEC in the environment in Burkina Faso.
机译：养牛业可通过废物，污水或牛粪便促进腹泻病传播。该研究旨在表征从牛粪，堆肥过程中的粪便和泥浆（从瓦加杜古的四个牛场收集）中分离的腹泻性大肠杆菌（DEC）。 2015年5月至2016年5月，共从四个牛市中收集了585个样品（堆肥过程中有340头牛粪，200种稀浆和45种粪肥）。多重聚合酶链反应（PCR），即16-plex PCR用于同时筛选生产志贺毒素的大肠杆菌（STEC），肠致病性大肠杆菌（EPEC），产肠毒素的大肠杆菌（ETEC），肠侵袭性大肠杆菌（EIEC）和肠聚集性大肠杆菌（EAEC）的特异性毒力基因）。在10.76％的样品中检测到DEC。 ETEC是最流行的（9.91％）。观察到STEC和EAEC的发生率相同（0.51％）。在堆肥过程中，在粪便中占12.64％，在粪便中占6.66％，在浆液中占5％，检测到ETEC。在堆肥过程中，在0.58％的牛粪便和2.22％的粪便中检测到STEC。在堆肥过程中，仅在1％的浆液和2.22％的粪便中检测到EAEC。基于estIa基因和/或estIb基因和/或elt基因扩增来鉴定ETEC菌株。在58个ETEC中，包含10.34％的astA，17.24％的包含elt，3.44％的包含estIa和79.31％的包含estIb。 EAEC的两个阳性菌株仅包含aggR基因，第三个仅对pic基因呈阳性。结果表明，来自牛市的废水可导致DEC在布基纳法索环境中的传播。

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