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首页> 外文期刊>International Journal of Environmental Research and Public Health >Genotoxic Changes to Rodent Cells Exposed in Vitro to Tungsten, Nickel, Cobalt and Iron
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Genotoxic Changes to Rodent Cells Exposed in Vitro to Tungsten, Nickel, Cobalt and Iron

机译：暴露于钨，镍，钴和铁的啮齿动物细胞的遗传毒性变化

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Tungsten-based materials have been proposed as replacements for depleted uranium in armor-penetrating munitions and for lead in small-arms ammunition. A recent report demonstrated that a military-grade composition of tungsten, nickel, and cobalt induced a highly-aggressive, metastatic rhabdomyosarcoma when implanted into the leg muscle of laboratory rats to simulate a shrapnel wound. The early genetic changes occurring in response to embedded metal fragments are not known. In this study, we utilized two cultured rodent myoblast cell lines, exposed to soluble tungsten alloys and the individual metals comprising the alloys, to study the genotoxic effects. By profiling cell transcriptomes using microarray, we found slight, yet distinct and unique, gene expression changes in rat myoblast cells after 24 h metal exposure, and several genes were identified that correlate with impending adverse consequences of ongoing exposure to weapons-grade tungsten alloy. These changes were not as apparent in the mouse myoblast cell line. This indicates a potential species difference in the cellular response to tungsten alloy, a hypothesis supported by current findings with in vivo model systems. Studies examining genotoxic-associated gene expression changes in cells from longer exposure times are warranted.
机译：有人建议使用钨基材料来替代穿甲弹药中的贫铀，并代替小武器弹药中的铅。最近的一份报告表明，军用级的钨，镍和钴成分植入到实验大鼠的腿部肌肉中以模拟弹片伤口时，会诱发高度攻击性的转移性横纹肌肉瘤。响应于嵌入的金属片段而发生的早期遗传变化是未知的。在这项研究中，我们利用两种培养的啮齿动物成肌细胞系，将它们暴露于可溶性钨合金和包含该合金的单个金属，以研究其遗传毒性作用。通过使用微阵列分析细胞转录组，我们发现暴露于金属24小时后大鼠成肌细胞中基因表达有轻微但独特的变化，并且鉴定了与持续暴露于武器级钨合金的潜在不良后果相关的几个基因。这些变化在小鼠成肌细胞系中并不明显。这表明细胞对钨合金的反应中存在潜在的物种差异，这一假设得到了体内模型系统当前发现的支持。必须进行研究，以研究长时间暴露于细胞中与遗传毒性相关的基因表达的变化。

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