...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Environmental Research and Public Health >Detection of Antibiotic Resistant Staphylococcus aureus from Milk: A Public Health Implication
【24h】

Detection of Antibiotic Resistant Staphylococcus aureus from Milk: A Public Health Implication

机译：牛奶中抗药性金黄色葡萄球菌的检测：对公共健康的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to investigate the occurrence, antibiotic susceptibility profiles, and virulence genes determinants of S. aureus isolated from milk obtained from retail outlets of the North-West Province, South Africa. To achieve this, 200 samples of raw, bulk and pasteurised milk were obtained randomly from supermarkets, shops and some farms in the North-West Province between May 2012 and April 2013. S. aureus was isolated and positively identified using morphological (Gram staining), biochemical (DNase, catalase, haemolysis and rapid slide agglutination) tests, protein profile analysis (MALDI-TOF mass spectrometry) and molecular (nuc specific PCR) methods. The antimicrobial resistance profiles of the isolates were determined using the phenotypic agar diffusion method. Genes encoding enterotoxins, exfoliative toxins and collagen adhesins were also screened using PCR. Among all the samples examined, 30 of 40 raw milk samples (75%), 25 of 85 bulk milk samples (29%) and 10 of 75 pasteurised milk samples (13%) were positive for S. aureus. One hundred and fifty-six PCR-confirmed S. aureus isolates were obtained from 75 contaminated milk samples. A large proportion (60%–100%) of the isolates was resistant to penicillin G, ampicillin, oxacillin, vancomycin, teicoplanin and erythromycin. On the contrary, low level resistance (8.3%–40%) was observed for gentamicin, kanamycin and sulphamethoxazole. Methicillin resistance was detected in 59% of the multidrug resistant isolates and this was a cause for concern. However, only a small proportion (20.6%) of these isolates possessed PBP2a which codes for Methicillin resistance in S. aureus. In addition, 32.7% of isolates possessed the sec gene whereas the sea, seb sed, see, cna, eta, etb genes were not detected. The findings of this study showed that raw, bulk and pasteurised milk in the North-West Province is contaminated with toxigenic and multi-drug resistant S. aureus strains. There is a need to implement appropriate control measures to reduce contamination as well as the spread of virulent S. aureus strains and the burden of disease in humans.
机译：这项研究的目的是调查从南非西北省的零售商店获得的牛奶中分离出的金黄色葡萄球菌的发生，抗生素敏感性概况和致病性基因决定因素。为了实现这一目标，在2012年5月至2013年4月之间，从西北省的一家超市，商店和一些农场中随机抽取了200份原料奶，散装奶和巴氏杀菌奶。分离了金黄色葡萄球菌，并用形态学（革兰氏染色）进行了阳性鉴定。 ，生化（DNase，过氧化氢酶，溶血和快速玻片凝集）测试，蛋白质谱分析（MALDI-TOF质谱）和分子（nuc特异性PCR）方法。使用表型琼脂扩散法确定分离物的抗药性。还使用PCR筛选了编码肠毒素，脱落性毒素和胶原粘附素的基因。在所有检查的样本中，40份原奶样本中的30份（占75％），85份散装牛奶样本中的25份（占29％）和75份巴氏灭菌牛奶样本中的10份（13％）对金黄色葡萄球菌呈阳性。从75个受污染的牛奶样品中获得了156个PCR确证的金黄色葡萄球菌分离株。分离株中很大一部分（60％–100％）对青霉素G，氨苄青霉素，奥沙西林，万古霉素，替考拉宁和红霉素有抗性。相反，庆大霉素，卡那霉素和磺胺甲恶唑的抗药性较低（8.3％–40％）。在59％的耐多药菌株中检出了耐甲氧西林，这引起了人们的关注。然而，这些分离株中只有一小部分（20.6％）具有PBP2a，其编码金黄色葡萄球菌的耐甲氧西林。此外，有32.7％的分离株具有sec基因，而未检测到海，硒，cna，eta和etb基因。这项研究的结果表明，西北省的原料奶，散装奶和巴氏杀菌奶被产毒和多药耐药的金黄色葡萄球菌菌株污染。需要采取适当的控制措施以减少污染以及金黄色葡萄球菌毒株的传播以及人类疾病的负担。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号