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首页> 外文期刊>International Journal of Agriculture and Biology >Genetic Diversity and Genetic Relationships of Amomum tsao-ko Based on Random Amplified Polymorphic DNA Markers
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Genetic Diversity and Genetic Relationships of Amomum tsao-ko Based on Random Amplified Polymorphic DNA Markers

机译：基于随机扩增多态性DNA标记的砂仁砂仁的遗传多样性和亲缘关系

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摘要

Amomum tsao-ko is a perennial herb of the ginger family. Fruit is used as a medicine and is often added to foods as a spice, but little information on its genetic features is currently available. In this study, 96 A. tsao-ko samples from eight populations were analyzed using RAPD molecular markers. Twelve RAPD primers produced 492 loci, 98.98% (487) of which were polymorphic. Marker index (MI) values and mean polymorphism information content (PIC) represented 8.095 and 0.197, respectively. At the population level, the percentage of polymorphic bands (PPB) was 57.18%, Nei's gene diversity (H) was 0.121, and Shannon's information index (I) was 0.202. The genetic differentiation coefficient among populations was Gst=0.084, which was similar to the 6% genetic variation among populations analyzed by AMOVA, indicating that there was no genetic differentiation among the populations of A. tsao-ko. A Mantel test (r = 0.153, P >0.05) further indicated that the geographic distance was not related to estimates of genetic distance. UMPGA cluster and principal coordinate analysis (PCoA) all divided 96 A. tsao-ko samples into three major groups.
机译：砂仁砂仁是生姜家族的多年生草本植物。水果被用作药物，通常作为香料添加到食品中，但是目前尚无有关其遗传特征的信息。在这项研究中，使用RAPD分子标记分析了来自八个种群的96株拟南芥。十二个RAPD引物产生492个基因座，其中98.98％（487）是多态的。标记指数（MI）值和平均多态信息含量（PIC）分别代表8.095和0.197。在人口水平上，多态性带（PPB）的百分比为57.18％，Nei的基因多样性（H）为0.121，Shannon的信息指数（I）为0.202。群体间的遗传分化系数为Gst = 0.084，与AMOVA分析得出的群体间6％的遗传变异相似，表明A.tsao-ko群体之间没有遗传分化。一项Mantel检验（r = 0.153，P> 0.05）进一步表明，地理距离与遗传距离的估计无关。 UMPGA聚类和主坐标分析（PCoA）均将96个拟南芥（A. tsao-ko）样本分为三个主要组。

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