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首页> 外文期刊>International Journal of Agriculture and Biology >Improving Regeneration from Mature Embryo Derived Callus in Wheat (Triticum aestivum) by Pre- and Post-callus Induction Treatments
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Improving Regeneration from Mature Embryo Derived Callus in Wheat (Triticum aestivum) by Pre- and Post-callus Induction Treatments

机译：愈伤组织诱导前和诱导后处理改善小麦成熟胚衍生愈伤组织的再生

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Immature embryos are preferred explant source to get regeneration frequency compatible for genetic transformation in wheat. Under natural conditions, immature embryos can be obtained only once a year. Either controlled conditions to grow wheat in off season should be developed or alternative explant should be optimized. To develop mature embryos as explant, two commercial wheat varieties “AARI-11” and “Galaxy-13” were used for the development of regeneration system. Seeds were imbibed with five different concentrations of 2,4-D for 24 h. Embryos were isolated from imbibed seeds, subjected to seven callus induction media (CIM) containing different commercial auxins, and regeneration was achieved on two regeneration media (RM). Effects of genotypes, 2,4-D in imbibition solutions, CIM, and their interactions were studied. Imbibition produced significant effects on callus induction and embryogenesis. Imbibition solution with 8 mg/L 2, 4-D and CIM4 (2,4-D+Dicamba as growth regulator) was found to be most favorable combination for callus induction, and embryogenesis. Both regeneration media responded excellent for regeneration with 81.10% and 80.62% regeneration frequency. Our results also showed that this is not only the regeneration media but also the genotype, CIM and imbibition solutions that played their role for embryogenic callus induction and regeneration. To further improve regeneration the effects of starvation stress, and extended time at CIM were analyzed for embryogenesis induction. The best embryogenesis was observed after 3 weeks of stress and 9 weeks old calli maintained on CIM through sub-culturing. ? 2017 Friends Science Publishers.
机译：未成熟的胚是优选的外植体来源，以获得与小麦遗传转化兼容的再生频率。在自然条件下，每年只能获得一次未成熟的胚胎。应该开发可控制的淡季小麦种植条件，或者优化外植体。为了发育成熟的胚作为外植体，使用了两个商业小麦品种“ AARI-11”和“ Galaxy-13”来开发再生系统。用五种不同浓度的2,4-D吸收种子24小时。从吸收的种子中分离出胚，将其置于含有不同商业生长素的七种愈伤组织诱导培养基（CIM）中，并在两种再生培养基（RM）上实现了再生。研究了基因型，2,4-D吸收溶液，CIM及其相互作用的影响。吸入对愈伤组织的诱导和胚发生产生显着影响。发现具有8 mg / L 2、4-D和CIM4（2,4-D +麦草畏为生长调节剂）的吸液是诱导愈伤组织和胚胎发生的最有利组合。两种再生介质均以81.10％和80.62％的再生频率对再生产生了出色的响应。我们的结果还表明，这不仅是再生培养基，而且是基因型，CIM和吸收溶液，它们在胚性愈伤组织的诱导和再生中发挥了作用。为了进一步改善再生，分析了饥饿胁迫的影响以及在CIM延长的时间诱导胚胎发生。通过传代培养在CIM上维持3周和9周大的愈伤组织后，观察到最佳的胚胎发生。 ？ 2017年《 Friends Science》出版社。

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