• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Implant Dentistry >Aspergillus fumigatus biofilm formation on different bone substitutes used in maxillary sinus augmentation: an in vitro analysis
【24h】

Aspergillus fumigatus biofilm formation on different bone substitutes used in maxillary sinus augmentation: an in vitro analysis

机译：烟熏曲霉生物膜在上颌窦增生中使用的不同骨替代物上的形成：体外分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract BackgroundFungus ball (FB) typically affects healthy adults, and Aspergillus fumigatus is the most frequent etiologic agent: iatrogenic factors represent an important issue in FB pathogenesis. Moreover, a recent study suggested a significant association between the use of anorganic bovine bone as sinus grafting material and subsequent development of FB. The aim of the present investigation is to evaluate in vitro eventual differences in the ability of Aspergillus fumigatus to colonize different bone grafting materials and grow on them as biofilm.FindingsFive different bone substitutes (demineralized bone matrix, anorganic bovine bone, ?-tricalcium phosphate, synthetic nano-hydroxyapatite, and synthetic hydroxyapatite), commonly used in sinus floor augmentation procedures, were inoculated with conidia suspensions of A. fumigatus and incubated at 37?°C for 4 and 8?h, in standardized conditions. Biofilm bound to the different materials underwent quantitative and qualitative analysis by confocal and scanning electron microscopy. A. fumigatus proved to be able to adhere and form biofilm on all the tested bone substitutes. The surface plot representation of the samples displayed some differences in the density of the superficial layer, due to the physical characteristics of the biomaterials. Nevertheless, Kruskal–Wallis test showed no significant differences in biomass amount among the five bone substitutes ( p =?0.236 and p =?0.55 after 4 and 8?h adhesion, respectively).ConclusionsAll the bone substitutes normally used in sinus floor augmentation represent a favorable substrate for fungal growth, due to their physical and chemical characteristics. During sinus floor elevation procedures, Schneiderian membrane integrity should be maintained in order to avoid the exposure of the grafting material at the respiratory environment, with potential risks of fungal colonization.
机译：摘要背景真菌球（FB）通常会影响健康的成年人，而烟曲霉是最常见的病原体：医源性因素是FB发病机理中的重要问题。此外，最近的一项研究表明，使用无机牛骨作为鼻窦移植材料与随后的FB形成之间存在显着关联。本研究的目的是评估烟曲霉在不同骨移植材料上定植并以生物膜形式在其上生长的能力的最终最终差异。五种不同的骨替代品（脱矿骨基质，无机牛骨，γ-磷酸三钙，通常在鼻窦底增大手术中使用的合成纳米羟基磷灰石和合成羟基磷灰石）被烟曲霉的分生孢子悬浮液接种，并在标准化条件下于37°C孵育4和8？h。通过共聚焦和扫描电子显微镜对结合到不同材料上的生物膜进行定量和定性分析。烟曲霉被证明能够在所有测试的骨替代物上粘附并形成生物膜。由于生物材料的物理特性，样品的表面图表示显示出表层密度的一些差异。尽管如此，Kruskal–Wallis试验显示，五个骨替代物之间的生物量没有显着差异（在粘附4和8h后分别为p =？0.236和p =？0.55）。结论所有通常用于窦底隆突的骨替代物都代表由于其物理和化学特性，是真菌生长的有利底物。在窦底抬高手术过程中，应保持施耐德膜的完整性，以避免移植材料暴露在呼吸环境中，并有潜在的真菌定植风险。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号