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首页> 外文期刊>International Journal of Medical Sciences >Effects of Selenium Yeast on Oxidative Stress, Growth Inhibition, and Apoptosis in Human Breast Cancer Cells
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Effects of Selenium Yeast on Oxidative Stress, Growth Inhibition, and Apoptosis in Human Breast Cancer Cells

机译：硒酵母对人乳腺癌细胞氧化应激，生长抑制和凋亡的影响

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Recent evidence suggests that selenium (Se) yeast may exhibit potential anti-cancer properties; whereas the precise mechanisms remain unknown. The present study was aimed at evaluating the effects of Se yeast on oxidative stress, growth inhibition, and apoptosis in human breast cancer cells. Treatments of ER-positive MCF-7 and triple-negative MDA-MB-231 cells with Se yeast (100, 750, and 1500 ng Se/mL), methylseleninic acid (MSA, 1500 ng Se/mL), or methylselenocysteine (MSC, 1500 ng Se/mL) at a time course experiment (at 24, 48, 72, and 96 h) were analyzed. Se yeast inhibited the growth of these cancer cells in a dose- and time-dependent manner. Compared with the same level of MSA, cancer cells exposure to Se yeast exhibited a lower growth-inhibitory response. The latter has also lower superoxide production and reduced antioxidant enzyme activities. Furthermore, MSA (1500 ng Se/mL)-exposed non-tumorigenic human mammary epithelial cells (HMEC) have a significant growth inhibitory effect, but not Se yeast and MSC. Compared with MSA, Se yeast resulted in a greater increase in the early apoptosis in MCF-7 cells as well as a lower proportion of early and late apoptosis in MDA-MB-231 cells. In addition, nuclear morphological changes and loss of mitochondrial membrane potential were observed. In conclusion, a dose of 100 to 1500 ng Se/mL of Se yeast can increase oxidative stress, and stimulate growth inhibitory effects and apoptosis induction in breast cancer cell lines, but does not affect non-tumorigenic cells.
机译：最近的证据表明硒酵母可能具有潜在的抗癌特性。而确切的机制仍然未知。本研究旨在评估硒酵母对人乳腺癌细胞氧化应激，生长抑制和细胞凋亡的影响。用硒酵母（100、750和1500 ng Se / mL），甲基硒酸（MSA，1500 ng Se / mL）或甲基硒代半胱氨酸（MSC）处理ER阳性MCF-7和三阴性MDA-MB-231细胞，在一个时程实验（在24、48、72和96小时）分析了1500 ng Se / mL）。硒酵母以剂量和时间依赖性的方式抑制这些癌细胞的生长。与相同水平的MSA相比，暴露于Se酵母的癌细胞表现出较低的生长抑制反应。后者还具有较低的超氧化物产生和降低的抗氧化酶活性。此外，暴露于MSA（1500 ng Se / mL）的非致瘤性人乳腺上皮细胞（HMEC）具有明显的生长抑制作用，而Se酵母和MSC没有。与MSA相比，Se酵母导致MCF-7细胞早期凋亡的增加更大，而MDA-MB-231细胞早期凋亡和晚期凋亡的比例降低。另外，观察到核形态变化和线粒体膜电位的损失。总之，硒酵母的100到1500 ng Se / mL剂量可以增加氧化应激，并刺激乳腺癌细胞系的生长抑制作用和凋亡诱导，但不影响非致瘤细胞。

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