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首页> 外文期刊>International Journal of Medical Sciences >Administration of CoCl2 Improves Functional Recovery in a Rat Model of Sciatic Nerve Transection Injury
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Administration of CoCl2 Improves Functional Recovery in a Rat Model of Sciatic Nerve Transection Injury

机译：在大鼠坐骨神经横断损伤模型中，CoCl2的使用改善了功能恢复

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Peripheral nerve injury is known to activate the hypoxia-inducible factor-1α (HIF-1α) pathway as one of pro-regenerative transcriptional programs, which could stimulate multiple injury-induced gene expression and contribute to axon regeneration and functional recovery. However, the role of HIF-1α in peripheral nerve regeneration remains to be fully elucidated. In this study, rats were divided into three groups and treated with sham surgery, surgery with cobalt chloride (CoCl2) and surgery with saline, respectively. Sciatic functional index, morphologic evaluations of muscle fibers, and never conduction velocity were performed to measure the functional recovery at 12 weeks postoperatively. In addition, the effects of CoCl2 on the expression of HIF-1α, glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) were determined at mRNA levels; as well as HIF-1α, the dual leucine zipper kinase (DLK), the c-Jun N-terminal kinase (JNK), phosphorylated JNK (p-JNK), BDNF and NGF were measured at protein level at 4 weeks postoperatively. Systemic administration of CoCl2 (15 mg/kg/day intraperitoneally) significantly promoted functional recovery of rats with sciatic nerve transection injury. This study demonstrated in rats treated with CoCl2, the expression of HIF-1α, GDNF, BDNF and NGF was significantly increased at mRNA level, while HIF-1α, DLK, p-JNK, BDNF and NGF was significantly increased at protein level.
机译：众所周知，周围神经损伤可激活缺氧诱导因子-1α（HIF-1α）途径，作为促再生转录程序之一，可刺激多种损伤诱导的基因表达，并促进轴突再生和功能恢复。但是，HIF-1α在周围神经再生中的作用仍有待充分阐明。在这项研究中，将大鼠分为三组，分别进行假手术，氯化钴（CoCl2）手术和盐水手术。进行坐骨神经功能指数，肌纤维形态学评估和永不传导速度，以测量术后12周的功能恢复情况。此外，在mRNA水平上测定了CoCl2对HIF-1α，神经胶质细胞系神经营养因子（GDNF），脑源性神经营养因子（BDNF）和神经生长因子（NGF）表达的影响。以及HIF-1α，在手术后4周时以蛋白水平检测双亮氨酸拉链激酶（DLK），c-Jun N端激酶（JNK），磷酸化JNK（p-JNK），BDNF和NGF。腹膜内给予CoCl2（15 mg / kg /天）可显着促进坐骨神经横断损伤大鼠的功能恢复。这项研究表明，在用CoCl2处理的大鼠中，HIF-1α，GDNF，BDNF和NGF的表达在mRNA水平上显着增加，而HIF-1α，DLK，p-JNK，BDNF和NGF在蛋白水平上显着增加。

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