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首页> 外文期刊>International journal of molecular imaging >In VivoTracking of Murine Adipose Tissue-Derived Multipotent Adult Stem Cells andEx VivoCross-Validation
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In VivoTracking of Murine Adipose Tissue-Derived Multipotent Adult Stem Cells andEx VivoCross-Validation

机译：体内小鼠脂肪组织多能成体干细胞的体内跟踪和体外交叉验证

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Stem cells are characterized by the ability to renew themselves and to differentiate into specialized cell types, while stem cell therapy is believed to treat a number of different human diseases through either cell regeneration or paracrine effects. Herein, anin vivoandex vivonear infrared time domain (NIR TD) optical imaging study was undertaken to evaluate the migratory ability of murine adipose tissue-derived multipotent adult stem cells [mAT-MASC] after intramuscular injection in mice.In vivoNIR TD optical imaging data analysis showed a migration of DiD-labelled mAT-MASC in the leg opposite the injection site, which was confirmed by a fibered confocal microendoscopy system.Ex vivoNIR TD optical imaging results showed a systemic distribution of labelled cells. Considering a potential microenvironmental contamination, a cross-validation study by multimodality approaches was followed: mAT-MASC were isolated from male mice expressing constitutively eGFP, which was detectable using techniques of immunofluorescence and qPCR. Y-chromosome positive cells, injected into wild-type female recipients, were detected by FISH. Cross-validation confirmed the data obtained byin vivo/ex vivoTD optical imaging analysis. In summary, our data demonstrates the usefulness of NIR TD optical imaging in tracking delivered cells, giving insights into the migratory properties of the injected cells.
机译：干细胞的特征在于能够自我更新并分化成专门的细胞类型，而干细胞疗法被认为可以通过细胞再生或旁分泌作用来治疗多种人类疾病。在本文中，进行了体内和体外的近红外时域（NIR TD）光学成像研究，以评估小鼠肌肉内注射后小鼠脂肪组织衍生的多能成体干细胞[mAT-MASC]的迁移能力。体内NIR TD光学成像数据分析结果显示DiD标记的mAT-MASC在与注射部位相对的小腿中迁移，这通过纤维共聚焦显微内窥镜系统得到了证实。离体NIR TD光学成像结果显示了标记细胞的全身分布。考虑到潜在的微环境污染，我们通过多模式方法进行了交叉验证研究：从表达组成型eGFP的雄性小鼠中分离出mAT-MASC，可使用免疫荧光和qPCR技术对其进行检测。通过FISH检测注入野生型雌性受体的Y染色体阳性细胞。交叉验证确认了通过体内/离体TD光学成像分析获得的数据。总而言之，我们的数据证明了NIR TD光学成像在追踪递送的细胞中的有用性，从而深入了解了所注射细胞的迁移特性。

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