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首页> 外文期刊>International journal of molecular imaging >Comparison of99mTc-Tetrofosmin and99mTc-Sestamibi Uptake in Glioma Cell Lines: The Role of P-Glycoprotein Expression
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Comparison of99mTc-Tetrofosmin and99mTc-Sestamibi Uptake in Glioma Cell Lines: The Role of P-Glycoprotein Expression

机译：胶质瘤细胞系中99mTc-四磷灵和99mTc-西他米比摄取的比较：P-糖蛋白表达的作用

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99mTc-Tetrofosmin (99mTc-TF) and99mTc-Sestamibi (99mTc-MIBI) are SPECT tracers that have been used for brain tumor imaging. Tumor’s multidrug resistance phenotype, namely, P-glycoprotein (p-gp), and the multidrug resistance related proteins (MRPs) expression have been suggested to influence both tracers’ uptake. In the present study we set out to compare99mTc-TF and99mTc-MIBI uptake in high-grade glioma cell lines and to investigate the influence of gliomas p-gp expression on both tracers’ uptake. We used four glioma cell lines (U251MG, A172, U87MG, and T98G). The expression of p-gp protein was evaluated by flow cytometry. TwentyμCi (7.4·105 Bq) of99mTc-TF and99mTc-MIBI were used. The radioactivity in the cellular lysate was measured with a dose calibrator. P-gp was significantly expressed only in the U251MG cell line (P<0.001). In all gliomas cell lines (U251MG, U87MG, A172, and T98G) the99mTc-TF uptake was significantly higher than99mTc-sestamibi. The U251MG cell line, in which significant p-gp expression was documented, exhibited the strongest uptake difference.99mTc-TF uptake was higher than99mTc-MIBI in all studied high-grade glioma cell lines. Thus,99mTc-TF may be superior to99mTc-MIBI for glioma imagingin vivo.
机译：99mTc-四磷磷酰胺（99mTc-TF）和99mTc-西沙比比（99mTc-MIBI）是已经用于脑肿瘤成像的SPECT示踪剂。肿瘤的多药耐药表型，即P-糖蛋白（p-gp）和多药耐药相关蛋白（MRPs）的表达已被建议影响两种示踪剂的摄取。在本研究中，我们着手比较高级神经胶质瘤细胞系中99mTc-TF和99mTc-MIBI的摄取，并研究神经胶质瘤p-gp表达对两种示踪剂摄取的影响。我们使用了四种神经胶质瘤细胞系（U251MG，A172，U87MG和T98G）。通过流式细胞术评估p-gp蛋白的表达。使用了20μCi（7.4·105 Bq）的99mTc-TF和99mTc-MIBI。用剂量校准器测量细胞裂解物中的放射性。 P-gp仅在U251MG细胞系中表达（P <0.001）。在所有神经胶质瘤细胞系（U251MG，U87MG，A172和T98G）中，99mTc-TF的摄取显着高于99mTc-司他巴比。在U251MG细胞系中，p-gp表达明显，其摄取差异最大。在所有研究的高级神经胶质瘤细胞系中，99mTc-TF摄取均高于99mTc-MIBI。因此，对于体内神经胶质瘤成像，99mTc-TF可能优于99mTc-MIBI。

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