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Expression of SRPK1 in gliomas and its role in glioma cell lines viability

机译：SRPK1在神经胶质瘤中的表达及其在神经胶质瘤细胞系中的作用

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摘要

Among factors regulating the splicing of major importance is serine/arginine protein kinase 1 (SRPK1) that phosphorylates SR splicing factors. SRPK1 is expressed in the mammalian central nervous system in a region- and neuron-specific manner. Based on previous observations that glial cells are practically devoid of SRPK1 and reports showing aberrant expression of SRPK1 in numerous tumors, but with conflicting roles, this study aims to investigate the expression of SRPK1 in glioma and its influence on tumor cell biological features. As shown by immunohistochemical analysis, malignant glioma cells express SRPK1 in glioblastomas with significant association between SRPK1 expression and patients' survival. SRPK1 expression was also significantly upregulated at the messenger RNA (mRNA) and protein level in glioma cell lines. Small interfering RNA-mediated downregulation of SRPK1 had little effect on cell viability, while it slightly enhanced the sensitivity of cells to killing by cisplatin. These results support the idea that at least in vitro, the effect of SRPK1 knockdown on the viability of glioma cell lines is rather limited, while the in vivo effects could be attributed to the modulation of angiogenesis by SRPK1.
机译：调节剪接的最重要因素是丝氨酸/精氨酸蛋白激酶1（SRPK1），它使SR剪接因子磷酸化。 SRPK1以区域和神经元特异性方式在哺乳动物中枢神经系统中表达。基于先前的观察，胶质细胞实际上缺乏SRPK1，并且有报道显示SRPK1在许多肿瘤中异常表达，但作用相互矛盾，因此本研究旨在研究SRPK1在神经胶质瘤中的表达及其对肿瘤细胞生物学特性的影响。如免疫组织化学分析所示，恶性神经胶质瘤细胞在胶质母细胞瘤中表达SRPK1，这与SRPK1的表达与患者的生存密切相关。在神经胶质瘤细胞系中，在信使RNA（mRNA）和蛋白质水平上，SRPK1表达也显着上调。小干扰RNA介导的SRPK1的下调对细胞活力几乎没有影响，而它稍微增强了细胞对顺铂杀伤的敏感性。这些结果支持这样的想法，即至少在体外，SRPK1敲低对神经胶质瘤细胞系活力的影响是相当有限的，而体内的作用可能归因于SRPK1对血管生成的调节。

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