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首页> 外文期刊>International journal of molecular medicine >Effect of advanced oxidation protein products on the proliferation and osteogenic differentiation of rat mesenchymal stem cells
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Effect of advanced oxidation protein products on the proliferation and osteogenic differentiation of rat mesenchymal stem cells

机译：先进的氧化蛋白产物对大鼠间充质干细胞增殖和成骨分化的影响

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Advanced oxidation protein products (AOPPs) as a novel marker of oxidative stress, are involved in a variety of diseases, including osteoporosis. Although a number of studies have shown the possible functions of AOPPs in biological processes, little is known about the role of AOPPs in the pathogenesis of osteoporosis. In this study, we aimed to investigate the effect of AOPPs on the proliferation and osteogenic differentiation of rat mesenchymal stem cells (MSCs). MSCs, isolated from bone marrow, were cultured in the absence or presence of AOPPs (50, 100, 200 and 400?mg/ml). MTT assay was used to determine the proliferative ability of the cells. Alkaline phosphatase (ALP) activity, the mRNA expression of ALP and collagen?I and bone nodule formation were detected to assess osteogenic differentiation. Reactive oxygen species (ROS) generation was analyzed with the probe 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA). The expression of receptor of advanced glycation end-products (RAGE) at the mRNA and protein level was detected by real-time PCR and western blot analysis, respectively. Compared with the control group, AOPPs inhibited MSC proliferation in a dose- and time-dependent manner. Moreover, AOPPs induced a significant reduction in ALP activity, as well as a decrease in ALP and collagen?I mRNA levels in the MSCs; bone nodule formation was also inhibited. Furthermore, AOPPs increased ROS generation in the MSCs, and upregulated the expression of RAGE at the mRNA and protein level. These results suggest that AOPPs inhibit the proliferation and osteogenic differentiation of MSCs, possibly through the AOPPs-RAGE-ROS pathway; this may be an important mechanism in the development of osteoporosis.
机译：先进的氧化蛋白产品（AOPP）作为氧化应激的新标记，涉及多种疾病，包括骨质疏松症。尽管许多研究表明了AOPPs在生物过程中的可能功能，但对AOPPs在骨质疏松症发病机理中的作用知之甚少。在这项研究中，我们旨在研究AOPPs对大鼠间充质干细胞（MSCs）增殖和成骨分化的影响。在不存在或存在AOPPs（50、100、200和400？mg / ml）的情况下，培养分离自骨髓的MSC。使用MTT测定法确定细胞的增殖能力。检测碱性磷酸酶（ALP）的活性，ALP和胶原蛋白I的mRNA表达以及骨结节的形成，以评估成骨分化。用探针2'，7'-二氯二氢荧光素二乙酸酯（DCFH-DA）分析了活性氧（ROS）的产生。实时荧光定量PCR和蛋白质印迹分析分别检测了晚期糖基化终产物（RAGE）的受体在mRNA和蛋白质水平的表达。与对照组相比，AOPPs以剂量和时间依赖性方式抑制MSC的增殖。此外，AOPPs诱导了MSC中ALP活性的显着降低，以及ALP和胶原I mRNA水平的降低。骨结节的形成也受到抑制。此外，AOPP增加了MSC中ROS的生成，并在mRNA和蛋白水平上调了RAGE的表达。这些结果表明，AOPPs可能通过AOPPs-RAGE-ROS途径抑制了MSC的增殖和成骨分化。这可能是骨质疏松症发展的重要机制。

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