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首页> 外文期刊>International Journal of Neonatal Screening >Glue for Manufacturing Heel Prick Filter Cards Does Not Interfere with the Measurement of Analytes for Newborn Screening
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Glue for Manufacturing Heel Prick Filter Cards Does Not Interfere with the Measurement of Analytes for Newborn Screening

机译：用于制造脚跟点刺滤卡的胶水不会干扰新生儿筛查的分析物的测量

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摘要

Population-based newborn screening (NBS) using blood collected and dried on filter paper was developed in the 1960s and remains the international standard for NBS programs. Glue, used in the manufacture of dried blood collection cards, may present a source of contamination and is often considered as a possible cause of anomalous results in routine screening. Our study evaluates this potential contamination on NBS analyses. EBF#1003 glue was blotted onto dried blood collection cards made of Whatman grade 903 filter paper (Whatman#903) and adult whole blood was pipetted onto the dried glue blots. In addition, blank glue blots (i.e., no blood) and dried blood spots (DBSs) in the absence of glue were prepared. The DBSs and blank samples were run in duplicate as routine samples for NBS. DBS absorption time and diameter, the effect of glue on measurements (concentrations and variation) were assessed. DBS absorption time and shape were equivalent for DBSs prepared in the absence and presence of undiluted glue. DBS diameter increased when prepared in the presence of glue. When EBF#1003 was diluted prior to use, DBS absorption time increased, and DBSs were non-uniform. Glue, diluted or not, did not produce measurements above the established Limit of Detection (LOD) for all assays used in the current Dutch screening programme. For all analytes with concentrations in the quantifiable range, contamination with glue had no effect on measurement variation, as it appeared equivalent to variation in untreated DBSs. Our data show that, in the unlikely event of contamination of Whatman#903 with EBF#1003, there is no effect on the measured concentration of analytes.
机译：1960年代开发了使用在滤纸上收集并干燥的血液进行的基于人口的新生儿筛查（NBS），并且仍然是NBS计划的国际标准。用于制造干血采集卡的胶水可能会带来污染，通常被认为是常规筛查结果异常的可能原因。我们的研究评估了NBS分析中的这种潜在污染。将EBF＃1003胶水吸干到由Whatman 903级滤纸（Whatman＃903）制成的干血采集卡上，然后将成人全血吸干到干胶印迹上。另外，在没有胶水的情况下，准备空白胶水印迹（即，没有血液）和干血斑（DBS）。 DBS和空白样品作为NBS的常规样品一式两份运行。评估了DBS的吸收时间和直径，胶水对测量的影响（浓度和变化）。在不存在和未稀释胶的情况下，DBS的吸收时间和形状与DBS相当。在有胶水的情况下制备时，DBS直径增加。使用前将EBF＃1003稀释后，DBS吸收时间增加，并且DBS不均匀。对于当前荷兰筛查计划中使用的所有测定法，稀释或未稀释的胶水的测量结果均未超过既定的检测限（LOD）。对于浓度在可量化范围内的所有分析物，胶水污染对测量值的变化没有影响，因为它似乎等同于未经处理的DBS的变化。我们的数据表明，万一EBF＃1003污染Whatman＃903时，对测得的分析物浓度没有影响。

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