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首页> 外文期刊>International Journal of Poultry Sciences >Satellite Cell Mitotic Activity of Broilers Fed Differing Levels of Lysine
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Satellite Cell Mitotic Activity of Broilers Fed Differing Levels of Lysine

机译：饲喂不同赖氨酸水平的肉鸡的卫星细胞有丝分裂活性

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Post-hatch myofiber growth is dependent upon the addition of new nuclei from the mitotically active satellite cell population. The objective of this study was to examine the relationship between different levels of dietary lysine and satellite cell mitotic activity during the early post-hatch period. Broiler chicks were split into five groups of 10 birds each immediately post-hatch. One group was not provided any feed or water for the first three days post-hatch, whereas the other groups were provided a standard starter diet with different levels of lysine (0.82, 0.99, 1.16, 1.33%) for the first three days post-hatch. All birds were injected with 5-Bromo-2`-deoxyuridine (BrdU) 2 hours before they were killed on the third day post-hatch. Mitotically active satellite cells were identified in the Pectoralis thoracicus and quantified using BrdU immunohistochemistry in combination with computer-based image analysis. Satellite cell mitotic activity was significantly (P < 0.05) lower in the starved compared to any of the fed groups. However, satellite cell mitotic activity was highest (P < 0.05) in the birds that were provided a lysine deficient diet (0.82%). The current study suggests that it is possible to nutritionally stimulate the satellite cell population in the early post-hatch chick, and that it is an important endeavour to re-examine the nutritional requirements of the early post-hatch chick to optimize meat yield.
机译：孵化后肌纤维的生长取决于有丝分裂活跃卫星细胞群体中新核的添加。这项研究的目的是检查孵化后早期饮食中不同水平赖氨酸与卫星细胞有丝分裂活性之间的关系。孵化后立即将雏鸡分为五组，每组十只。一组在孵化后的前三天没有提供任何饲料或水，而另一组在孵化后的前三天提供了具有不同赖氨酸水平（0.82、0.99、1.16、1.33％）的标准发酵剂饮食。孵化。在孵化后第三天将所有禽类杀死前2小时，给它们注射5-溴2`-脱氧尿苷（BrdU）。在胸大肌中鉴定出具有线粒体活性的卫星细胞，并使用BrdU免疫组织化学结合基于计算机的图像分析对其进行定量。与任何喂养组相比，饥饿的卫星细胞有丝分裂活性显着降低（P <0.05）。然而，在赖氨酸缺乏饮食的家禽中，卫星细胞的有丝分裂活性最高（P <0.05）。目前的研究表明，有可能在孵化后早期雏鸡中营养刺激卫星细胞群，并且这是一项重要的工作，重新检查孵化后早期雏鸡的营养需求以优化肉的产量。

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