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首页> 外文期刊>International Journal of Poultry Sciences >Effects of Strain and Production-Cycle on Indices of Lysine Catabolism in Turkeys
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Effects of Strain and Production-Cycle on Indices of Lysine Catabolism in Turkeys

机译：应变和生产周期对土耳其赖氨酸分解代谢指标的影响

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In typical turkey production diets, lysine is frequently the second limiting amino acid . Understanding its catabolism could provide opportunities to increase the efficiency of lysine use for protein synthesis. We hypothesize that indices of lysine catabolism in turkey liver vary throughout the production cycle. Two commercial strains of turkey, Strain A and B, were analyzed 8 times over a period of 17 weeks (n = 8 birds/sampling time/strain) for lysine alpha-ketoglutarate (LKR) and saccharopine dehydrogenase (SDH) activities and mRNA abundance, in vitro lysine oxidation (LOX), L- amino acid oxidase (LAAO) activity, lysyl oxidase (LO) activity and mRNA expression of cationic amino acid transporters (CAT) 1, 2 and 3. We found differences in hepatic LKR activity (p = 0.007), LKR mRNA (p = 0.0004), SDH activity (p = 0.008), LOX (p<0.0001), lysyl oxidase activity (p<0.01), LAAO (p<0.0001) and CAT1 (p = 0.005) and 2 mRNA abundance (p = 0.0022) throughout the production cycle. We also found an effect of strain on SDH (p = 0.046) and CAT-2 mRNA abundance (p = 0.02); while no age or strain effects were detected for CAT3 mRNA abundance. Interestingly, the average LKR and SDH activities across strains and weeks was 240 and 420 nmol per minute per gram of liver, respectively, as opposed to the average LAAO activity, lysyl oxidase activity and L OX which were 0.70, 0.10 and 13.5 nmol per minute per gram liver, respectively. These data indicate that the saccharopine-dependent pathway is the predominant pathway of lysine degradation in turkey liver and that indices of hepatic lysine catabolism vary throughout the production cycle.
机译：在典型的火鸡生产饮食中，赖氨酸经常是第二限制性氨基酸。了解其分解代谢可能会提供机会来提高赖氨酸用于蛋白质合成的效率。我们假设火鸡肝脏中赖氨酸分解代谢的指标在整个生产周期中都不同。在17周内（n = 8只禽/采样时间/株），对土耳其的两个商业株A和B进行了8次分析，分析其赖氨酸α-酮戊二酸（LKR）和糖精脱氢酶（SDH）活性以及mRNA丰度，体外赖氨酸氧化（LOX），L-氨基酸氧化酶（LAAO）活性，赖氨酰氧化酶（LO）活性和阳离子氨基酸转运蛋白（CAT）1、2和3的mRNA表达。我们发现肝LKR活性存在差异（ p = 0.007），LKR mRNA（p = 0.0004），SDH活性（p = 0.008），LOX（p <0.0001），赖氨酰氧化酶活性（p <0.01），LAAO（p <0.0001）和CAT1（p = 0.005）在整个生产周期中有2个mRNA丰度（p = 0.0022）。我们还发现菌株对SDH（p = 0.046）和CAT-2 mRNA丰度（p = 0.02）的影响; CAT3 mRNA丰度未发现年龄或品系影响。有趣的是，各个菌株和数周的平均LKR和SDH活性分别为每克肝脏每分钟240和420 nmol每分钟，而平均LAAO活性，赖氨酰氧化酶活性和L OX分别为每分钟0.70、0.10和13.5 nmol每克肝脏。这些数据表明，糖醋平依赖性途径是土耳其肝脏赖氨酸降解的主要途径，并且肝脏赖氨酸分解代谢的指标在整个生产周期中都不同。

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