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首页> 外文期刊>International Journal of Poultry Sciences >Association of NRAMP1 Polymorphisms with Immune Traits in Indonesian Native Chickens
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Association of NRAMP1 Polymorphisms with Immune Traits in Indonesian Native Chickens

机译：印尼本地鸡NRAMP1基因多态性与免疫性状的关联。

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Background: Natural resistance-associated macrophage protein-1 gene ( NRAMP1 ) plays an important role in immune response against intracellular pathogens. Objective: This study was aimed at identifying NRAMP1 polymorphisms and their association with immune traits in Indonesian native chickens. Methodology: Genetic polymorphism of chickens was investigated using polymerase chain reaction-restriction fragment polymorphism. The concentrations of leukocytes and differentiation (heterophile, lymphocyte and monocyte) were assessed by the Giemsa method and immune traits were detected by followed clearance test. Results: The results showed that NRAMP1 was polymorphic in all native chickens. The CC genotype was significantly higher than CT and TT genotypes (p<0.05) in Sentul chickens resistant to Salmonella pullorum . Although, the concentrations of leukocytes and differentiation in chickens with all three of NRAMP1 genotypes (CC, CT and TT) were not statistically different, there was a significant correlation between different NRAMP1 genotypes and immune traits. Therfore, NRAMP1 is proposed to be a disease resistance candidate gene. Conclusion: However, this study should be validated in other chicken populations to evaluate the potential exploitation of NRAMP1 in selective breeding.
机译：背景：天然抗性相关的巨噬细胞蛋白1基因（NRAMP1）在针对细胞内病原体的免疫应答中起着重要作用。目的：本研究旨在鉴定印度尼西亚本土鸡的NRAMP1多态性及其与免疫性状的关系。方法：使用聚合酶链反应-限制性片段多态性研究了鸡的遗传多态性。通过吉姆萨方法评估白细胞浓度和分化（嗜异性细胞，淋巴细胞和单核细胞），并通过随后的清除测试检测免疫性状。结果：结果表明，NRAMP1在所有天然鸡中均具有多态性。在抗白痢沙门氏菌的森图尔鸡中，CC基因型显着高于CT和TT基因型（p <0.05）。尽管三种NRAMP1基因型（CC，CT和TT）的鸡中白细胞浓度和分化均无统计学差异，但不同NRAMP1基因型与免疫性状之间存在显着相关性。因此，NRAMP1被提议为抗病候选基因。结论：然而，该研究应在其他鸡群中进行验证，以评估NRAMP1在选择性育种中的潜在开发。

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