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Polymerase Chain Reaction

机译:聚合酶链反应

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The polymerase chain reaction (PCR) is a technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. There are three major steps involved in the PCR technique: denaturation, annealing and extension. PCR is useful in the investigation and diagnosis of a growing number of diseases. PCR is also used in forensics laboratories. PCR can identify genes that have been implicated in the development of cancer. The present paper is an attempt to review basics of PCR in relation to its methods, application and use.
机译:聚合酶链反应(PCR)是分子生物学中的一种技术,可以跨数个数量级扩增DNA的单个或几个拷贝,从而生成特定DNA序列的数千到数百万个拷贝。现在,PCR是医学和生物学研究实验室用于各种应用的一种常见且通常不可缺少的技术。 PCR技术涉及三个主要步骤:变性,退火和延伸。 PCR可用于调查和诊断越来越多的疾病。 PCR还用于法医实验室。 PCR可以鉴定与癌症发展有关的基因。本文是对PCR的基础,方法,应用和使用进行综述的尝试。

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