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首页> 外文期刊>International Journal of Scientific & Technology Research >In Vitro Efficacy Of Lactic Acid Bacteria With Antifungal Activity Against Fusarium Sp. CID124-CS Isolate From Chilli Seeds
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In Vitro Efficacy Of Lactic Acid Bacteria With Antifungal Activity Against Fusarium Sp. CID124-CS Isolate From Chilli Seeds

机译：乳酸菌对镰刀菌抗真菌活性的体外研究辣椒种子的CID124-CS分离株

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Lactic acid bacteria (LAB) are known as to have inhibitory activity against fungi and other pathogens. In this study LAB from soil and fermented chilli fruits were evaluated for their inhibitory activity against Fusarium sp. CID124-CS that was isolated from chilli fruits. Three LAB isolates (Lb. plantarum1-MSS, P. pentoceous1-MSS isolated from soil, one Lb. plantarum1-FCF from fermented chilli) and two from ATCC culture Lb. acidophilus ATTCC314 and Lb. plantarum ATCC8014 showed strong inhibitory activity against growth of target fungi evaluated by well diffusion assay showed high growth inhibition (6.05 mm to 7.60 mm) within 48 h at 28oC. Adding LAB supernatant to Potato Dextrose Broth (PDA) with fungi reduced mycelia growth from 36.00% to 60.00%. Similarly, fungal mass reduction with cells of LAB in De Man Rogosa and Sharpe Broth (MRSB) ranged 98.0% to 99.9% after 72 h incubation at 28oC by micro tire plate assay. Whereas, conidial germination in MEB with LAB supernatants were reduced by 93.3% to 96.6% using micro titre plate assay. This study showed that cells and CFS of LAB isolated from soil have antifungal activity and could be used as antifungal agent against Fusarium sp. CID124-CS that infect to chilli.
机译：乳酸菌（LAB）已知对真菌和其他病原体具有抑制活性。在这项研究中，评估了土壤和发酵辣椒果实中的LAB对镰孢镰刀菌的抑制活性。从辣椒果实中分离出来的CID124-CS。 3种LAB分离株（从土壤中分离的Lb. plantarum1-MSS，P。pentoceous1-MSS，一种从发酵辣椒中分离的Lb. plantarum1-FCF）和2种从ATCC培养物Lb中分离。嗜酸性ATTCC314和Lb。通过良好的扩散分析评估，车前草ATCC8014对目标真菌的生长具有很强的抑制活性，在28oC下48 h内显示出较高的生长抑制作用（6.05 mm至7.60 mm）。将LAB上清液添加到带有真菌的马铃薯葡萄糖肉汤（PDA）中，可使菌丝体生长从36.00％降低到60.00％。同样，在28°C下通过微型轮胎板测定，在De Man Rogosa和Sharpe Broth（MRSB）中的LAB细胞对真菌的抑制作用范围为98.0％至99.9％。相比之下，使用微量滴定板测定法，带有LAB上清液的MEB中的分生孢子萌发减少了93.3％至96.6％。这项研究表明，从土壤中分离出的LAB细胞和CFS具有抗真菌活性，可用作抗镰刀菌的抗真菌剂。感染辣椒的CID124-CS。

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