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首页> 外文期刊>International Journal of Scientific World >Investigating prevalence of pathogenic genes (ETA and TSST-1) in Staphylococcus aureus isolated from different wards of the hospitals by PCR method
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Investigating prevalence of pathogenic genes (ETA and TSST-1) in Staphylococcus aureus isolated from different wards of the hospitals by PCR method

机译：应用PCR方法调查医院不同病区分离出的金黄色葡萄球菌致病基因（ETA和TSST-1）的流行情况

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Staphylococcus aureus is the most common pathogenic organisms in the hospitals and communities' infections. It is responsible for more than 80 percent of infectious diseases. The purpose of the present paper is to determine the incidence of Staphylococcus pathogenic genes isolated from different wards of hospitals by PCR method. This study included 61 Staphylococcus aureusisolates collected from different wards hospital, between 2011 and 2012 in University of Kurdistan (Toohhid and Besat hospitals). All isolates were previously identified as Staphylococcus aureusby a standard microbiological procedure. It isolates were incubated at 37? for 24h on blood agar; single colonies were tested with tube and slide coagulase, catalase tests and growth on Manito salt agar. Following genomic DNA extraction, the presence of ETA, TSST-1 genes was analyzed by PCR.61 strains of Staphylococcus aureushave been isolated from different wards of the hospital. Frequency of tst gene was 81% and eta gene was 47%. Moreover, frequency of strains with both eta and tst genes was 40%. Results of the present paper indicate that the prevalence of Staphylococcusaureus results on prevalence of eta and tst genes, and this is a matter of concern.
机译：金黄色葡萄球菌是医院和社区感染中最常见的致病菌。它负责80％以上的传染病。本文的目的是通过PCR方法确定从医院不同病房分离出的葡萄球菌致病基因的发生率。这项研究包括2011年至2012年在库尔德斯坦大学（Toohhid和Besat医院）从不同病房医院收集的61株金黄色葡萄球菌。以前，所有分离株均已通过标准微生物程序鉴定为金黄色葡萄球菌。将其分离物在37℃温育。在血琼脂上放置24小时;用管和玻片凝固酶，过氧化氢酶测试以及在Manito盐琼脂上的生长测试单个菌落。在提取基因组DNA之后，通过PCR分析了ETA，TSST-1基因的存在。从医院的不同病房分离出61株金黄色葡萄球菌菌株。 tst基因的频率为81％，而eta基因的频率为47％。此外，同时具有eta和tst基因的菌株的频率为40％。结果表明，金黄色葡萄球菌的流行与eta和tst基因的流行有关，这是一个值得关注的问题。

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