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首页> 外文期刊>International Journal of Nanomedicine >Targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA
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Targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA

机译：靶向肺癌治疗：转铁蛋白修饰的纳米结构脂质载体的制备和优化，作为新型纳米药物，可共同递送抗癌药物和DNA

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Purpose: Nanostructured lipid carriers (NLC) represent an improved generation of lipid nanoparticles. They have specific nanostructures to accommodate drugs/genes, and thus achieve higher loading capacity. The aim of this study was to develop transferrin (Tf)-decorated NLC as multifunctional nanomedicine for co-delivery of paclitaxel (PTX) and enhanced green fluorescence protein plasmid.Methods: Firstly, Tf-conjugated ligands were synthesized. Secondly, PTX- and DNA-loaded NLC (PTX-DNA-NLC) was prepared. Finally, Tf-containing ligands were used for the surface decoration of NLC. Their average size, zeta potential, drug, and gene loading were evaluated. Human non-small cell lung carcinoma cell line (NCl-H460 cells) was used for the testing of in vitro transfection efficiency, and in vivo transfection efficiency of NLC was evaluated on mice bearing NCl-H460 cells.Results: Tf-decorated PTX and DNA co-encapsulated NLC (Tf-PTX-DNA-NLC) were nano-sized particles with positive zeta potential. Tf-PTX-DNA-NLC displayed low cytotoxicity, high gene transfection efficiency, and enhanced antitumor activity in vitro and in vivo.Conclusion: The results demonstrated that Tf-PTX-DNA-NLC can achieve impressive antitumor activity and gene transfection efficiency. Tf decoration also enhanced the active targeting ability of the carriers to NCl-H460 cells. The novel drug and gene delivery system offers a promising strategy for the treatment of lung cancer.
机译：目的：纳米结构脂质载体（NLC）代表了脂质纳米颗粒的改进生成。它们具有特定的纳米结构以容纳药物/基因，从而实现更高的负载能力。这项研究的目的是开发修饰转铁蛋白（Tf）的NLC作为多功能纳米药物，用于共递送紫杉醇（PTX）和增强型绿色荧光蛋白质粒。方法：首先，合成Tf缀合的配体。其次，制备了载有PTX和DNA的NLC（PTX-DNA-NLC）。最后，将含Tf的配体用于NLC的表面装饰。评价了它们的平均大小，ζ电位，药物和基因负载。用人非小细胞肺癌细胞系（NCl-H460细胞）检测体外转染效率，并评估NLC对荷有NCl-H460细胞的小鼠的体内转染效率。结果：用Tf修饰的PTX和DNA共包裹的NLC（Tf-PTX-DNA-NLC）是具有正Zeta电位的纳米级颗粒。 Tf-PTX-DNA-NLC在体外和体内均显示出低细胞毒性，高基因转染效率和增强的抗肿瘤活性。结论：结果表明，Tf-PTX-DNA-NLC可实现令人印象深刻的抗肿瘤活性和基因转染效率。 Tf修饰还增强了载体对NC1-H460细胞的主动靶向能力。新型药物和基因递送系统为肺癌的治疗提供了有希望的策略。

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