• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Science and Qualitative Analysis >The Impact of Enzymes in the Hepatic Function
【24h】

The Impact of Enzymes in the Hepatic Function

机译：酶对肝功能的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study is the application of Alkaline Phosphatase (ALP) and Gamma-glutamyl Transferase (GGT) activity, by using the corresponding kinetic methods, at the Clinical Chemistry Laboratories in our place. Enzymes are proteins synthesized in the cells of living organisms in order to play the role of bio-catalysts. Today are recognized about 2000 different enzymes. Some of those have been isolated in pure form of the chemical. We used serum as a material and we took into consideration 15 healthy patients, 15 patients who had viral hepatitis and 15 patients with idiopathic hyper-bilirubinemia. Solution where the substance is being analyzed, was treated with specific reagents according to the procedures such as incubation, heating, etc, until the color is formed. The absorbance of the coloring solution was measured using photometry at a wavelength of 405nm. As an analytical method for the determination of ALP, we chose 4-nitrophenylphosphate (4NPP) as a substrate, while as a buffer for the optimal pH adjustment of the reaction we used 2-amino-2 methyl -1-propanol (AMP). ALP was within permitted values from 100 to 290 U /l, whereas Gamma-glutamyl Transferase was at normal reference values of 5 to 51 U /l. Automated method was equal to the standard method, with a correlation coefficient of r = 0997. Accuracy within the series of measurements had a variation coefficient of only 1.25%. From the results taken from the laboratories, we had an increased precision, accuracy, sensitivity and reliability as a result of the technological process of electronic engineering of new modern techniques with monoclonal antibodies and immobilized enzymes.
机译：这项研究的目的是通过相应的动力学方法，在我们临床化学实验室中应用碱性磷酸酶（ALP）和γ-谷氨酰转移酶（GGT）活性。酶是在活生物体的细胞中合成的蛋白质，可发挥生物催化剂的作用。今天公认大约有2000种不同的酶。其中一些已经以化学纯形式分离出来。我们使用血清作为材料，并考虑了15例健康患者，15例病毒性肝炎患者和15例特发性高胆红素血症患者。将要分析物质的溶液根据特定的试剂（例如温育，加热等）处理，直到形成颜色。使用光度法在405nm的波长下测量着色溶液的吸光度。作为测定ALP的分析方法，我们选择了4-硝基苯基磷酸酯（4NPP）作为底物，而为了优化反应pH的缓冲液，我们使用了2-氨基-2甲基-1-丙醇（AMP）。 ALP在100至290 U / l的允许值内，而Gamma-谷氨酰转移酶的正常参考值在5至51 U / l的范围内。自动化方法与标准方法相同，相关系数为r =0997。一系列测量的准确性的变异系数仅为1.25％。从实验室获得的结果，由于采用单克隆抗体和固定化酶的现代新技术的电子工程技术流程，我们提高了精度，准确性，灵敏性和可靠性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号