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首页> 外文期刊>International journal of soil science >Comparison between UV-spectrophotometry and HPLC Methods to Determine Napropamide Concentration in Soil Sorption Experiment
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Comparison between UV-spectrophotometry and HPLC Methods to Determine Napropamide Concentration in Soil Sorption Experiment

机译：紫外分光光度法与高效液相色谱法测定土壤吸附实验中丙酰胺浓度的比较

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In this study we compared the High Performance Liquid Chromatography (HPLC-UV) and the Ultraviolet (UV) spectrophotometry methods for measuring the herbicide napropamide concentrations in soil solution during batch equilibrium sorption study. Experiments were conducted to compare the calibration curves, recovery in background solution and spiked soil samples, intra and inter day precision and mass balance of the two methods. Mass balance of napropamide in the sorption study was determined from the difference between the initial napropamide content in the supernatant and the equilibrium concentration. Although DOC interfered with UV wave absorption by napropamide in the UV-spectrophotometry method, it was easily corrected by using a blank solution containing the same amount and type of DOC as used in the sample. The intra and inter day precision experiments indicated the measured napropamide concentrations using both methods were equally reproducible and consistent. The recovery of napropamide from spiked soil samples decreased with increasing amount of clay and organic matter but recoveries for both methods exceeded 88% and the recovery of the UV-spectrophotometry method was comparable to the HPLC. The measured concentrations of napropamide in soil solution supernatant used for the sorption study showed no significant differences between the two methods. We found that the UV-spectrophotometry method was reliable to determine the concentration of napropamide in the supernatant of the soil solution used in the sorption study. Therefore, the UV-spectrophotometry method can be used as an alternative method to HPLC for determination of napropamide concentration in soil batch equilibrium sorption study.
机译：在这项研究中，我们比较了高效液相色谱（HPLC-UV）和紫外（UV）分光光度法在间歇平衡吸附研究期间测量土壤溶液中除草剂萘丙酰胺浓度的方法。进行了实验以比较两种方法的校准曲线，背景溶液和加标土壤样品的回收率，日内和日间精度和质量平衡。吸附研究中萘丙酰胺的质量平衡由上清液中初始萘丙酰胺含量与平衡浓度之间的差异确定。尽管在紫外分光光度法中，DOC干扰了萘丙酰胺对UV波的吸收，但通过使用空白溶液（包含与样品中所用的DOC数量和类型相同的空白溶液）可以轻松地对其进行校正。日内和日间精密度实验表明，使用两种方法测得的萘丙酰胺浓度均具有相同的重现性和一致性。加标土壤样品中萘丙酰胺的回收率随粘土和有机物含量的增加而降低，但两种方法的回收率均超过88％，紫外分光光度法的回收率与HPLC相当。用于吸附研究的土壤溶液上清液中萘丙酰胺的测定浓度在两种方法之间没有显着差异。我们发现，紫外分光光度法可以可靠地测定吸附研究中所用土壤溶液上清液中的萘普胺浓度。因此，紫外分光光度法可作为测定土壤批料平衡吸附研究中萘胺浓度的高效液相色谱法的替代方法。

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