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首页> 外文期刊>International Journal of Tissue Engineering >Biomanufacturing versus Superficial Cell Seeding: Simulation of Chondrocyte Proliferation in a Cylindrical Cartilage Scaffold
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Biomanufacturing versus Superficial Cell Seeding: Simulation of Chondrocyte Proliferation in a Cylindrical Cartilage Scaffold

机译：生物制造与表面细胞播种：圆柱软骨支架中软骨细胞增殖的模拟。

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Local volume averaging approach was used for modeling and simulation of cell growth and proliferation, as well as glucose transfer within a cylindrical cartilage scaffold during cell cultivation. The scaffold matrix including the nutrient solution filling spaces among seeded cell colonies was treated as a porous medium. Applying differential mass balance of cells and glucose to a representative elementary volume of the scaffold, two diffusional mass transfer models were developed based on local volume averaged properties. The derived governing equations take into account time-dependent glucose diffusion, glucose consumption by cells, cell migration, apoptosis, and cell reproduction within the scaffold. Since the volumetric fraction of cells in the scaffold relies on cell growth, which strongly depends on glucose concentration in the scaffold, the governing equations were solved simultaneously using implicit finite difference method and Gauss-Seidel technique. Simulation results showed that cell volumetric fraction of the scaffold can reach about 45% after 50 days if a culture medium with a glucose concentration of 45 kgm−3is used. Also, simulation results indicate that more uniform and higher average cell volume fraction of the scaffold can be obtained if biomanufacturing-based cell seeding is used across the scaffold rather than cell seeding on the scaffold surface.
机译：局部体积平均法用于细胞培养过程中细胞生长和增殖以及圆柱形软骨支架内的葡萄糖转移的建模和仿真。将包括种子细胞集落之间的营养液填充空间的支架基质视为多孔介质。将细胞和葡萄糖的不同质量平衡应用于支架的代表性基本体积，基于局部体积平均性质开发了两个扩散质量转移模型。推导的控制方程式考虑了时间依赖性葡萄糖扩散，细胞葡萄糖消耗，细胞迁移，凋亡和支架内细胞繁殖。由于支架中细胞的体积分数取决于细胞生长，而细胞生长在很大程度上取决于支架中的葡萄糖浓度，因此使用隐式有限差分法和高斯-塞德尔技术同时求解了控制方程。仿真结果表明，如果使用葡萄糖浓度为45 kgm-3的培养基，支架的细胞体积分数在50天后可以达到约45％。而且，模拟结果表明，如果跨支架使用基于生物制造的细胞接种而不是在支架表面上进行细胞接种，则可以获得支架的更均匀和更高的平均细胞体积分数。

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