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首页> 外文期刊>International Journal of Tissue Engineering >90° Peel off Tests of Tissue Engineered Osteochondral Constructs: A New Method to Determine the Osteochondral Integration
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90° Peel off Tests of Tissue Engineered Osteochondral Constructs: A New Method to Determine the Osteochondral Integration

机译：组织工程骨软骨构建体的90°剥离测试：确定骨软骨整合的新方法

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One promising treatment of localized osteochondral defects in the knee joint may be the implantation of in vitro tissue-engineered osteochondral constructs. A crucial aspect of this kind of osteochondral construct is the bonding between the bone-scaffold and scaffold-based chondral layer. Here, a 90° peel off test is proposed as an appropriate method to measure the integration of cartilage to bone in osteochondral constructs for different primary methods of bonding the cartilage scaffold to the bone scaffold, with and without seeded chondroblasts. The method was developed and then tested on tissue-engineered constructs. The force/displacement data obtained allow determination of both the maximum force and the total energy required to separate the two layers. The tests showed good reproducibility and good discrimination between measurements as a function of seeding times. Average maximum peel-off forces varied between 10 mN for fibrin glue only to 575 mN for constructs with cells after four weeks of incubation. Linear regression of the area under the curve (AUC) as a function of maximum force shows a high correlation between the two parameters withR2 = 0.97. The main limitation of the test is that the data provide only a modest ability to decide how uniform the bond is over the area between the two layers.
机译：膝关节局部骨软骨缺损的一种有希望的治疗方法可能是植入体外组织工程化的骨软骨构建体。这种骨软骨构造的关键方面是骨支架和基于支架的软骨层之间的结合。在这里，提出了一种90°剥离测试，作为测量软骨支架与骨支架结合到骨支架（带有或不带有播种的成软骨细胞）的不同主要方法的合适方法，以测量软骨在骨软骨构造中与骨的整合。开发了该方法，然后在组织工程结构上进行了测试。获得的力/位移数据允许确定最大力和分离两层所需的总能量。这些测试显示出良好的可重复性，并且根据播种时间对测量值进行了很好的区分。孵育四周后，平均最大剥离力在纤维蛋白胶的10 mN和仅在有细胞的构建体的575 mN之间变化。曲线下面积（AUC）作为最大力的函数的线性回归表明，两个参数之间的相关性很高，R2 = 0.97。该测试的主要局限性在于，数据仅提供适度的能力来确定两层之间区域上的键的均匀程度。

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