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首页> 外文期刊>International Journal of Virology >Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein
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Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein

机译：编码运动蛋白中枢部分的RNA2中的葡萄扇叶病毒和拟南芥花叶病毒分离株之间的序列差异

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In this study, we reported sequencing and molecular analysis in detail of central part of 2BMP gene (approximately 270 nts) in case of Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). For more complex analysis of genetic variability we added reference sequences of evaluated nepoviruses and also sequence of phylogenetically very similar Nepovirus GDefV. Range of sequence analysis showed 74.5-99.3% identity at the nucleotide level and 86.5-100% identity at the amino acid level for partial gene 2BMP among Czech, Italian, German, Swiss sequences and references of all isolates. In this study eleven isolates were sequenced consisting of 5 GFLV and 6 ArMV isolates. As expected, GFLV and ArMV sequences were distinguished based on sequence identities but differences were not significant. Surprisingly, GDefV sequence was almost identical with the majority of GFLV sequences, even more than some GFLV sequences between each other. Local complexity of nucleotide sequences was also evaluated and it was in average of 0.530 for GFLV and GDefV, for ArMV the average value was 0.533. This study provides detail insights for the genetic variability of GFLV, ArMV and GDefV in the central part of the 2BMP coding region.
机译：在这项研究中，我们报道了在葡萄扇叶病毒（GFLV）和阿拉比斯花叶病毒（ArMV）的情况下2B MP 基因中心部分（约270 nts）的详细测序和分子分析。为了更复杂地分析遗传变异性，我们添加了评估的nepovirus的参考序列，以及系统发育上非常相似的nepovirus GDefV的序列。序列分析范围显示部分基因2B MP 在捷克，意大利，德国，瑞士和所有参考文献中的核苷酸水平上的同源性为74.5-99.3％，氨基酸水平上的同源性为86.5-100％。隔离株。在这项研究中，对11个分离株进行了测序，其中包括5个GFLV和6个ArMV分离株。正如预期的那样，GFLV和ArMV序列是基于序列同一性进行区分的，但差异并不明显。令人惊讶的是，GDefV序列与大多数GFLV序列几乎相同，甚至比彼此之间的某些GFLV序列更多。还评估了核苷酸序列的局部复杂性，GFLV和GDefV的平均值为0.530，ArMV的平均值为0.533。这项研究为2B MP 编码区中心部分的GFLV，ArMV和GDefV的遗传变异提供了详细的见识。

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