• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Internet Journal of Hematology >Acute Toxicity and Blood Profile of Adult Clarias gariepinus Exposed to Lead Nitrate
【24h】

Acute Toxicity and Blood Profile of Adult Clarias gariepinus Exposed to Lead Nitrate

机译：暴露于硝酸铅的成年枝aria的急性毒性和血型

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Changes in Clarias gariepinus' blood cells were investigated after 96-h of exposure to lead. Ninety (90) Clarias gariepinus with average weight of 262.2g and average length of 30.8 were divided into 5 groups (A-E) at six (6) fish per group and in triplicates after being acclimatized for 14 days. They were then exposed to various concentrations (0, 25, 50, 100 and 200 mg/l) of lead nitrate for 96 hours. The 96-hour LC 50 was calculated to be 72.92; the packed cell volume (PCV) of the treatments decreased significantly relative to that of the control, while their platelet counts increased compared with the control. There was also a reduction in the RBC of treatments. Other blood parameters did not vary significantly in comparison to the control group, but it is worth noting that the mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) increased considerably in all treatments compared to the control. These alterations have been attributed to direct or feedback responses of structural damage to RBC membranes resulting in haemolysis and impairment in haemoglobin synthesis, stress related release of RBCs from the spleen and hypoxia, induced by exposure to lead. This study therefore gives an insight into toxic effect of acute exposure to lead on Clarias gariepinus. Introduction Environmental stressors and their associated risks have always been an inherent part of society. Aquatic ecosystems are especially sensitive to exposure to toxic contaminants. Pollutants either individually or in combination may have sub-lethal effects at the cellular, organ and individual level. The count of red blood cells is quite a stable index and the fish body tries to maintain this count within the limits of certain physiological standards using various physiological mechanisms of compensation. Studies have shown that when the water quality is affected by toxicants, any physiological changes will be reflected in the values of one or more of the haematological parameters (Van Vuren, 1986). Blood cell responses are important indicators of changes in the internal and/or external environment of animals. In fish, exposure to chemical pollutants can induce either increases or decreases in haematological levels. Their changes depend on fish species, age, the cycle of the sexual maturity of spawners and diseases (Golovina, 1996; Luskova, 1997). Like in warm-blooded animals, changes in the blood parameters of fish, which occur because of injuries of the latter organs or tissues, can be used to determine and confirm the dysfunction or injuries of the latter (organs or tissue). However in the fish, these parameters are more related to the response of the whole organism, i.e. to the effect on fish survival, reproduction and growth. It should be noted that although the mechanisms of fish physiology and biochemical reaction to xenobiotics has not been investigated enough, it is obvious that species differences of these mechanisms exist.Fish live in very intimate contact with their environment, and are therefore very susceptible to physical and chemical changes which may be reflected in their blood components (Wilson and Taylor, 1993). In fish, exposure to chemical pollutants can induce either increases or decreases in haematological levels. Blood tissue truly reflects physical and chemical changes occurring in organism; therefore, detailed information can be obtained on general metabolism and physiological status of fish in different groups of age and habitat. Early diagnosis is also possible when evaluating haematological data, particularly blood parameters (Folmar, 1993; Golovina, 1996; Luskova, 1997). Furthermore, it should be noted that haematological indices are of different sensitivity to various environmental factors and chemicals (Lebedeva et al, 1998; Vosylien?, 1999a, b). Previous haematological study of nutritional effects (Rehulka, 2000), infectious diseases (Rehulka, 2002a) and pollutants
机译：接触铅96小时后，研究了Cl草的血细胞变化。将平均重量为262.2g，平均长度为30.8的九十个（90）苦竹（Clarias gariepinus）分成5组（A-E），每组六（6）条鱼，在适应14天后一式三份。然后将它们暴露于各种浓度（0、25、50、100和200 mg / l）的硝酸铅中96小时。 96小时的LC 50计算为72.92;与对照组相比，处理后的包装细胞体积（PCV）显着下降，而血小板计数却增加。治疗的RBC也减少了。与对照组相比，其他血液参数没有显着变化，但值得注意的是，与对照组相比，所有治疗的平均红细胞体积（MCV），平均红细胞血红蛋白（MCH）和平均红细胞血红蛋白浓度（MCHC）均显着增加。控制。这些改变被归因于对红细胞膜的结构损伤的直接或反馈反应，导致溶血和血红蛋白合成受损，与铅相关的应激相关的红细胞从脾脏的释放和缺氧。因此，本研究提供了对铅的急性接触对Cl草的毒性作用的见解。引言环境压力源及其相关风险一直是社会的固有组成部分。水生生态系统对接触有毒污染物特别敏感。污染物单独或组合使用可能在细胞，器官和个体水平上产生亚致死作用。红细胞的数量是一个非常稳定的指标，鱼体试图通过各种生理补偿机制将其数量保持在某些生理标准的范围内。研究表明，当水质受到有毒物质的影响时，任何生理变化都会反映在一种或多种血液学参数的值上（Van Vuren，1986）。血细胞反应是动物内部和/或外部环境变化的重要指标。在鱼类中，接触化学污染物会导致血液学水平的升高或降低。它们的变化取决于鱼类的种类，年龄，产卵者的性成熟周期和疾病（Golovina，1996; Luskova，1997）。像在温血动物中一样，由于后一种器官或组织的损伤而导致的鱼类血液参数的变化可用于确定并确认后者（器官或组织）的功能障碍或损伤。但是，在鱼类中，这些参数与整个生物体的反应更相关，即与对鱼类生存，繁殖和生长的影响有关。应当指出的是，尽管尚未充分研究鱼类对异生物素的生理学和生化反应的机制，但很明显，这些机制存在物种差异。鱼与环境密切接触，因此很容易受到物理影响以及可能反映在其血液成分中的化学变化（Wilson和Taylor，1993）。在鱼类中，接触化学污染物会导致血液学水平的升高或降低。血液组织真正反映了生物体内发生的物理和化学变化;因此，可以获得有关不同年龄和生境的鱼类的一般代谢和生理状态的详细信息。在评估血液学数据，尤其是血液参数时，也可以进行早期诊断（Folmar，1993; Golovina，1996; Luskova，1997）。此外，应该注意的是，血液学指标对各种环境因素和化学物质的敏感性不同（Lebedeva等，1998; Vosylien？，1999a，b）。营养作用（Rehulka，2000），传染病（Rehulka，2002a）和污染物的血液学研究

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号