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首页> 外文期刊>Investigative Genetics >High throughput DNA sequencing to detect differences in the subgingival plaque microbiome in elderly subjects with and without dementia
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High throughput DNA sequencing to detect differences in the subgingival plaque microbiome in elderly subjects with and without dementia

机译：高通量DNA测序可检测患有和不患有痴呆的老年受试者牙龈下菌斑微生物组的差异

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Background To investigate the potential association between oral health and cognitive function, a pilot study was conducted to evaluate high throughput DNA sequencing of the V3 region of the 16S ribosomal RNA gene for determining the relative abundance of bacterial taxa in subgingival plaque from older adults with or without dementia. Methods Subgingival plaque samples were obtained from ten individuals at least 70?years old who participated in a study to assess oral health and cognitive function. DNA was isolated from the samples and a gene segment from the V3 portion of the 16S bacterial ribosomal RNA gene was amplified and sequenced using an Illumina HiSeq1000 DNA sequencer. Bacterial populations found in the subgingival plaque were identified and assessed with respect to the cognitive status and oral health of the participants who provided the samples. Results More than two million high quality DNA sequences were obtained from each sample. Individuals differed greatly in the mix of phylotypes, but different sites from different subgingival depths in the same subject were usually similar. No consistent differences were observed in this small sample between subjects separated by levels of oral health, sex, or age; however a consistently higher level of Fusobacteriaceae and a generally lower level of Prevotellaceae was seen in subjects without dementia, although the difference did not reach statistical significance, possibly because of the small sample size. Conclusions The results from this pilot study provide suggestive evidence that alterations in the subgingival microbiome are associated with changes in cognitive function, and provide support for an expanded analysis of the role of the oral microbiome in dementia.
机译：背景技术为了研究口腔健康与认知功能之间的潜在联系，进行了一项初步研究，以评估16S核糖体RNA基因V3区的高通量DNA测序，以确定患有或患有老年人的龈下菌斑中细菌类群的相对丰度。没有痴呆。方法从十名年龄至少70岁的个体中获取龈下菌斑样品，这些个体参加了一项评估口腔健康和认知功能的研究。从样品中分离出DNA，并使用Illumina HiSeq1000 DNA测序仪对16S细菌核糖体RNA基因V3部分的基因片段进行扩增和测序。确定并评估提供样本的参与者的认知状况和口腔健康，对龈下斑块中发现的细菌种群进行评估。结果从每个样品中获得了超过200万个高质量的DNA序列。个体在系统类型上的差异很大，但是在同一受试者中来自不同龈下深度的不同部位通常相似。在这个小样本中，没有观察到被口腔健康，性别或年龄分开的受试者之间的一致性差异。然而，在没有痴呆症的受试者中，梭菌科的水平一直较高，而原核科的水平通常较低，尽管由于样本量较小，差异没有统计学意义。结论这项初步研究的结果提供了暗示性的证据，表明龈下微生物组的改变与认知功能的变化有关，并为扩大口腔微生物组在痴呆症中的作用提供了支持。

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