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首页> 外文期刊>International Scholarly Research Notices >Escherichia coliATCC 8739 Adapts to the Presence of Sodium Chloride, Monosodium Glutamate, and Benzoic Acid after Extended Culture
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Escherichia coliATCC 8739 Adapts to the Presence of Sodium Chloride, Monosodium Glutamate, and Benzoic Acid after Extended Culture

机译：大肠杆菌ATCC 8739适应扩展培养后的氯化钠，谷氨酸钠和苯甲酸的存在

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Escherichia coliis commonly found in intestine of human, and any changes in their adaptation or evolution may affect the human body. The relationship betweenE. coliand food additives is less studied as compared to antibiotics.E. coliwithin our human gut are consistently interacting with the food additives; thus, it is important to investigate this relationship. In this paper, we observed the evolution ofE. colicultured in different concentration of food additives (sodium chloride, benzoic acid, and monosodium glutamate), singly or in combination, over 70 passages. Adaptability over time was estimated by generation time and cell density at stationary phase. Polymerase chain reaction (PCR)/restriction fragments length polymorphism (RFLP) using 3 primers and restriction endonucleases, each was used to characterize adaptation/evolution at genomic level. The amplification and digestion profiles were tabulated and analyzed by Nei-Li dissimilarity index. Our results demonstrate thatE. coliin every treatment had adapted over 465 generations. The types of stress were discovered to be different even though different concentrations of same additives were used. However, RFLP shows a convergence of genetic distances, suggesting the presence of global stress response. In addition, monosodium glutamate may be a nutrient source and support acid resistance inE. coli.
机译：大肠埃希氏菌通常在人的肠道中发现，其适应性或进化的任何变化都可能影响人体。 E之间的关系。与抗生素相比，大肠杆菌和食品添加剂的研究较少。人类肠道内的大肠杆菌一直与食品添加剂相互作用;因此，研究这种关系很重要。在本文中，我们观察了E的演变。在70代中单独或组合使用不同浓度的食品添加剂（氯化钠，苯甲酸和味精）进行大肠菌培养。通过固定时间的产生时间和细胞密度估计随时间的适应性。聚合酶链反应（PCR）/限制性片段长度多态性（RFLP）使用3个引物和限制性核酸内切酶，分别用于表征基因组水平的适应/进化。扩增和消化图谱被制表并通过Nei-Li不相似指数分析。我们的结果证明。大肠菌素的每种治疗方法已适应465代以上。即使使用不同浓度的相同添加剂，也发现应力类型不同。但是，RFLP显示出遗传距离的收敛，表明存在整体应激反应。此外，谷氨酸钠可能是营养来源，并支持E中的耐酸性。大肠杆菌。

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