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首页> 外文期刊>Iranian Journal Of Allergy, Asthma and Immunology >TH1 and TH2 Responses are Influenced by HLA Antigens in Healthy Neonates Vaccinated with Recombinant Hepatitis B Vaccine
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TH1 and TH2 Responses are Influenced by HLA Antigens in Healthy Neonates Vaccinated with Recombinant Hepatitis B Vaccine

机译：在健康的新生儿中接种重组乙型肝炎疫苗后，TH1和TH2反应受到HLA抗原的影响。

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The immune response to hepatitis B surface antigen (HBsAg) is influenced by several factors, of which HLA antigens and balanced secretion of Th1/Th2 cytokines play important roles. The aim of this study was to evaluate the influence of HLA antigens on cytokine secretion by HBsAg-stimulated peripheral blood mononuclear cells (PBMC) from healthy neonates vaccinated with recombinant HBsAg.Peripheral blood mononuclear cells (PBMC) were isolated from 48 Iranian neonates vaccinated with a recombinant HBV vaccine. The cells were stimulated in vitro with rHBsAg and the concentration of IL-4, IL-10, IL-12 and IFN-γ were quantitated in culture supernatant by sandwich ELISA. HLA typing was performed by microlymphocytotoxicity method.Significant diminished secretion of both Th1 (IFN-γ) and Th2 (IL-4, IL-10) cytokines was observed in HBsAg-stimulated PBMC from vaccinees expressing the HLA-DR7 compared to DR7 negative vaccinees. Similarly, lower production of these cytokines was also observed in vaccinees with DR7-DR53-DQ2, B7-DR7-DR53-DQ2 and A2-DR7-DR53-DQ2 haplotypes (p<0.05, p <0.005). While HBsAg-stimulated PBMC of DR13+ subjects produced lower levels of Th2-type cytokines (IL-4 and IL-10), those of HLA-B8+ or HLA-A9+ subjects produced higher levels of Th2-type cytokines. Cytokine secretion in response to PHA mitogen was not associated with a given HLA antigen or haplotype and was similarly represented in all groups of subjects irrespective of their HLA complex.These results indicate that HLA antigens may differentially influence cytokine secretion by HBsAg-specific T-cells of healthy neonates vaccinated with recombinant HB vaccine. This phenomenon may have an important implication for control of the immune response to HBsAg vaccine.?Key words: Hepatitis B vaccine, HLA, TH1, TH2, Cytokine
机译：对乙型肝炎表面抗原（HBsAg）的免疫反应受多种因素影响，其中HLA抗原和Th1 / Th2细胞因子的平衡分泌起重要作用。本研究的目的是评估HLA抗原对HBsAg刺激的健康新生儿的HBsAg刺激的外周血单个核细胞（PBMC）分泌细胞因子分泌的影响。从48例接受HBsAg免疫的伊朗新生儿中分离外周血单个核细胞（PBMC）。重组HBV疫苗。用rHBsAg体外刺激细胞，并通过夹心ELISA定量培养上清液中的IL-4，IL-10，IL-12和IFN-γ的浓度。通过微淋巴细胞毒性方法进行HLA分型。与DR7阴性疫苗相比，在HBsAg刺激的PBMC中，表达HLA-DR7的疫苗中Th1（IFN-γ）和Th2（IL-4，IL-10）细胞因子的分泌显着减少。 。同样，在具有DR7-DR53-DQ2，B7-DR7-DR53-DQ2和A2-DR7-DR53-DQ2单倍型的疫苗中也观察到这些细胞因子的产量较低（p <0.05，p <0.005）。 HBsAg刺激的DR13 +受试者的PBMC产生较低水平的Th2型细胞因子（IL-4和IL-10），而HLA-B8 +或HLA-A9 +受试者的PBMC产生较高水平的Th2型细胞因子。响应PHA丝裂原的细胞因子分泌与给定的HLA抗原或单倍型无关，并且在所有受试者组中均相似，无论其HLA复合物如何。这些结果表明HLA抗原可能会差异性地影响HBsAg特异性T细胞分泌细胞因子重组HB疫苗接种的健康新生儿的数量。该现象可能对控制HBsAg疫苗的免疫反应具有重要意义。关键词：乙型肝炎疫苗HLA TH1 TH2细胞因子

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