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首页> 外文期刊>Iranian Journal of Basic Medical Sciences >EFFECTS OF 1, 25-DIHYDROXYVITAMIN D3 ON IL-17/IL-23 AXIS, IFN-γ AND IL-4 EXPRESSION IN SYSTEMIC LUPUS ERYTHEMATOSUS INDUCED MICE MODEL
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EFFECTS OF 1, 25-DIHYDROXYVITAMIN D3 ON IL-17/IL-23 AXIS, IFN-γ AND IL-4 EXPRESSION IN SYSTEMIC LUPUS ERYTHEMATOSUS INDUCED MICE MODEL

机译：1，25-羟维生素D3对系统性红斑狼疮诱导的小鼠模型IL-17 / IL-23轴，IFN-γ和IL-4表达的影响

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Objective(s): Systemic lupus erythematosus (SLE) is a multi-factorial autoimmune disease which may be characterized by T lymphocytes dysfunctions. Th17 cells have been identified as new effector cells, which play an important role in the pathogenesis. In recent years, immunomodulatory effect of vitamin D3 has been noticed. In the present experiment, the effect of vitamin D3 on the expression of IL-17, IL-23, IL-4 and IFN- g were assessed in activated chromatin-induced mouse model for SLE.Materials and Methods: Five groups of mice were included in this study; Group one received active chromatin+CFA+PBS; Group 2 received vitamin D3 starting 2 weeks before disease induction; Group 3 received vitamin D3 (50 ng/day) starting with the disease establishment; Group 4 received non active chromatin+CFA+PBS; Group 5 received CFA+PBS. On day 56 splenocytes were isolated and gene expression of interleukin IL-17, IL-23, IL-4 and IFN- g were analyzed by Real-Time PCR method. Proteinuria and serum anti-dsDNA and Th17 levels were measured using commercial kits.Results: The results showed that IL-17, IL-23, and IFN- g mRNA expression, and IL-17 titers were decreased remarkably and that of IL-4 increased in mice which received vitamin D3 before SLE induction. Administration of vitamin D3 after the establishment of SLE failed to affect the IL-17 or IL-23 mRNA levels. Lastly, pre-treatment of mice with vitamin D3 decreased the anti-ds DNA antibody titer.Conclusion: Our findings showed that vitamin D3 supplementation in lupus induced mice through modulating the expression rate of some inflammatory cytokines diminished the inflammatory conditions in SLE.
机译：目的：系统性红斑狼疮（SLE）是一种多因素自身免疫性疾病，其特征可能是T淋巴细胞功能异常。 Th17细胞已被鉴定为新的效应细胞，在发病机理中起着重要作用。近年来，已经注意到维生素D3的免疫调节作用。在本实验中，在活化的染色质诱导的SLE小鼠模型中评估了维生素D3对IL-17，IL-23，IL-4和IFN-g表达的影响。材料与方法：五组小鼠包括在这项研究中;第一组接受活性染色质+ CFA + PBS。第2组在诱发疾病前2周开始接受维生素D3;第3组从疾病确定开始就接受维生素D3（50 ng /天）;第4组接受非活性染色质+ CFA + PBS;第5组接受CFA + PBS。在第56天，分离脾细胞，并通过实时PCR方法分析白介素IL-17，IL-23，IL-4和IFN-g的基因表达。结果：结果表明，IL-17，IL-23，IFN-g mRNA表达和IL-17滴度显着下降，IL-4和IL-4下降。在SLE诱导前接受维生素D3的小鼠体内维生素A含量增加。 SLE建立后服用维生素D3无法影响IL-17或IL-23 mRNA水平。最后，用维生素D3预处理小鼠降低了抗ds DNA抗体的滴度。

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