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Detection of Leptospires serogroups, Which Are Common Causes of Human Acute Leptospirosis in Guilan, Northern Iran

机译:钩端螺旋体血清群的检测,这是伊朗北部桂兰市人类急性钩端螺旋体病的常见原因

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Background:This study is performed to reveal most common species and subspecies of leptospires that are main causes of human leptospirosis in Guilan, Northern Province of Iran.Methods:We performed IgM-ELISA and MAT on 282 blood samples from patients who attended to 3 hospitals in the flat area of Guilan Province with clinical symptoms consisted with leptospirosis. All specimens with titers ≥ 160 against at least one pathogenic strain in MAT and with titers ≥160 in IgM-ELISA were regarded confirmed positive cases indicative acute disease. For any confirmed positive cases, we determined the strains, which had the highest titer to determine the frequency of most common serovars and serogroups.Results:Seventy of 282 sera had titers ≥160 against at least one pathogenic strain in MAT and titers ≥ 160 in IgM-ELISA. We determined frequency of common causative serogroups which had highest titers in 70 positive cases and only cases which had high titers in MAT and in IgM-ELISA were selected which is a reliable criterion to detect acute disease and to determine causative serogroup.Conclusion:Nine serogroups including sejroe, grippotyphosa, mini, ictero haemorrhagiae, celledoni, autumnalis, cynopteri, pomona, and javanica were more responsible of acute leptospirosis in Guilan.
机译:背景:本研究旨在揭示伊朗人北部省圭兰市人钩端螺旋体病主要病因的钩端螺旋体的最常见物种和亚种。方法:我们对来自3家医院就诊患者的282份血样进行了IgM-ELISA和MAT在桂兰平地,临床症状为钩端螺旋体病。所有对至少一种致病菌株在MAT中效价≥160且在IgM-ELISA中效价≥160的标本均被视为证实为急性疾病的阳性病例。对于任何确诊的阳性病例,我们确定了具有最高滴度的菌株,以确定最常见的血清和血清群的频率。结果:282份血清中有70份针对MAT中至少一种致病菌株的效价≥160,而在MAT中的效价≥160。 IgM-ELISA。我们确定了70例阳性病例中具有最高滴度的常见致病血清群的频率,仅选择了在MAT和IgM-ELISA中具有高滴度的病例,这是检测急性疾病和确定致病性血清群的可靠标准。结论:九个血清群包括sejroe,hippotyphosa,mini,黄疸出血,celledoni,autallalis,cynopteri,pomona和javanica在桂兰的急性钩端螺旋体病发病率更高。

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