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首页> 外文期刊>Italian journal of animal science >Increased adipocyte differentiation may be mediated by extracellular calcium levels through effects on calreticulin and peroxisome proliferator activated receptor gamma expression in intramuscular stromal vascular cells isolated from Hanwoo beef cattle
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Increased adipocyte differentiation may be mediated by extracellular calcium levels through effects on calreticulin and peroxisome proliferator activated receptor gamma expression in intramuscular stromal vascular cells isolated from Hanwoo beef cattle

机译：脂肪细胞分化的增加可能是通过影响钙质网蛋白和过氧化物酶体增殖物激活的受体γ在韩羽肉牛肌肉内基质血管细胞中的表达而介导的

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Marbling is the intramuscular deposition of adipose tissue and is said to be directly associated with beef quality. Nutritional manipulations are a common practice among beef cattle producers in order to achieve better quality meat. The objective of the current study was to investigate the effects of increasing extracellular calcium on the differentiation of intramuscular stromal vascular cells (IM SVCs) isolated from Hanwoo beef cattle. Primary cell isolates of SVCs were differentiated for 14 days while exposed to increasing concentrations (1.8, 3.6, 7.2, 10.8?mM) of calcium in the media. The correlation between adipogenesis and calcium concentrations was determined through SVC differentiation monitored by RT-PCR, Western blot analysis for PPARγ, C/EBPα, calreticulin, FABP4 and GLUT4 expression. In addition, expression of both phospholipase C gamma (PLC-γ) and protein kinase C (PKC) were investigated since both have possible links to intracellular calcium increase and the expression of several adipogenic genes including FABP4 and GLUT4. Results of the current study provide evidence that stromal vascular cells exposed to the lower concentrations of extracellular calcium have higher rates of adipogenesis possibly due to a decrease in the expression of calreticulin, a known inhibitor of PPARγ expression, and PKC activation followed by PLC-γ activation, leading to the expression of adipogenic genes. Data derived from this study shows that in vitro, decreasing calcium levels present in the microenvironment of fat precursor cells lead to a higher percentage of adipogenesis.
机译：大理石花纹是脂肪组织的肌肉内沉积，据说与牛肉质量直接相关。为了获得更好的肉质，营养操纵是肉牛生产者的普遍做法。本研究的目的是研究增加细胞外钙对分离自Hanwoo肉牛的肌内基质血管细胞（IM SVC）分化的影响。 SVC的原代细胞分离株分化14天，同时暴露于培养基中浓度不断增加的钙（1.8、3.6、7.2、10.8？mM）中。脂肪形成与钙浓度之间的相关性通过RT-PCR监测的SVC分化，PPARγ，C /EBPα，钙网蛋白，FABP4和GLUT4表达的Western印迹分析来确定。此外，研究了磷脂酶Cγ（PLC-γ）和蛋白激酶C（PKC）的表达，因为它们都可能与细胞内钙的增加以及包括FABP4和GLUT4在内的几种成脂基因的表达有关。目前的研究结果表明，暴露于较低浓度细胞外钙的基质血管细胞具有较高的脂肪生成速率，这可能是由于钙网蛋白的表达降低，钙网蛋白是一种已知的PPARγ表达抑制剂，PKC活化随后是PLC-γ激活，导致成脂基因的表达。这项研究得出的数据表明，在体外，脂肪前体细胞微环境中存在的钙水平降低导致脂肪形成的百分比更高。

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