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首页> 外文期刊>itleEngenharia ambiental: pesquisa e tecnologia >PHENOL DETERMINATION IN ENVIRONMENTAL INTEREST SAMPLES BY AN AMPEROMETRIC BIOSENSOR BASED ON LYOPHILIZED FUNGI TISSUE (Agaricus bisporus)
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PHENOL DETERMINATION IN ENVIRONMENTAL INTEREST SAMPLES BY AN AMPEROMETRIC BIOSENSOR BASED ON LYOPHILIZED FUNGI TISSUE (Agaricus bisporus)

机译：基于冻融真菌组织（双孢蘑菇）的安培生物传感器测定环境样品中的苯酚

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摘要

A biosensor based on lyophilized fungi tissue (Agaricus bisporus) was used for the amperometric determination of phenol in samples of environmental interest. This fungi tissue contained the tyrosinase enzyme that is able to catalyze two sequential oxidation reactions with phenolic substrates. Both reactions involve molecular oxygen, therefore the Clark-type oxygen electrode commercial was selected as transducer. Two samples of environmental interest were analyzed by the biosensor: a sample of domestic wastewater and a sample of feed water of a membrane bioreactor (MBR) tank, provided by General Electric Company. The results of the biosensor were compared with the results of the Standard Method for phenol determination, colorimetric method. The application of the biosensor in the samples showed percentage difference of 2.29 for the domestic wastewater and of 6.95 for the feed water when compared to the standard method, which shows a promising future to the biosensor.
机译：基于冻干真菌组织（双孢蘑菇）的生物传感器用于安培法测定环境样品中的苯酚。该真菌组织包含酪氨酸酶，该酪氨酸酶能够催化与酚类底物的两个连续氧化反应。两种反应都涉及分子氧，因此选择了Clark型氧电极作为传感器。通过生物传感器分析了两个对环境感兴趣的样品：由通用电气公司提供的生活污水样品和膜生物反应器（MBR）罐的给水样品。将生物传感器的结果与苯酚测定的标准方法，比色法的结果进行比较。与标准方法相比，样品中生物传感器的应用表明生活废水的百分比差异为2.29，给水百分比差异为6.95，这表明该生物传感器的前景广阔。

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