首页> 外国专利> SYSTEM AND METHOD FOR MEASURING SULPHITE IN FOOD SAMPLES USING AN AMPEROMETRIC BIOSENSOR AND THE USE OF SAID BIOSENSOR

SYSTEM AND METHOD FOR MEASURING SULPHITE IN FOOD SAMPLES USING AN AMPEROMETRIC BIOSENSOR AND THE USE OF SAID BIOSENSOR

机译：使用安培生物传感器测量食品样品中亚硫酸盐的系统和方法以及所述生物传感器的使用

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摘要

The invention relates to a system for measuring sulphite in food samples using a biosensor, a method for determining sulphite in food samples using said biosensor, and the use of said biosensor for measuring the sulphite values in food samples. The system uses an amperometric biosensor comprising a bi-enzymatic system, a working electrode, a counter electrode and a reference electrode. The bi-enzymatic system is composed of the human sulphite oxidase and peroxidase enzymes, and the immobilisation of the enzymes in the working electrode is carried out via simple physical retention. The mixture of the enzymes is mixed with a chemical mediator. The method comprises the following phases: applying a sample to the amperometric biosensor; changing the electrical current upon coming into contact with the analyte to be detected; and amperometrically measuring said electrical current in solutions with continuous agitation, using a constant potential.

机译：本发明涉及使用生物传感器测量食物样品中亚硫酸盐的系统，使用所述生物传感器确定食物样品中亚硫酸盐的方法，以及所述生物传感器用于测量食物样品中亚硫酸盐值的用途。该系统使用安培生物传感器，该传感器包括双酶系统，工作电极，反电极和参比电极。双酶系统由人亚硫酸盐氧化酶和过氧化物酶组成，这些酶在工作电极中的固定是通过简单的物理保留来实现的。酶的混合物与化学介质混合。该方法包括以下阶段：将样品施加到电流型生物传感器;以及将样品施加到电流传感器。与要检测的被分析物接触时改变电流;使用恒定电势，在连续搅拌的溶液中安培测量所述电流。

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公开/公告号EP3321670A4

专利类型
公开/公告日2019-01-02

原文格式PDF
申请/专利权人 BIOLAN MICROBIOSENSORES S.L.;
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申请/专利号EP16820885.8
发明设计人 GONZALEZ RIOJA ROBERTO;MAZA DEL RIO SONIA;SALLERES ALONSO SANDRA;JAUREGUIBEITIA CAYROLS ARRATE;GONZALEZ URQUIDI IRUNE;ARANTZAMENDI EGIGUREN ALAI;
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申请日2016-04-19
分类号G01N27/327;C12Q1/26;C12Q1/28;C12Q1;C12M1/34;C12M1/40;C12N9/08;G01N33/02;
国家 EP
入库时间 2022-08-21 12:28:14

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