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首页> 外文期刊>Iranian Journal of Microbiology >Characterization of Afb, a novel bifunctional protein in Streptococcus agalactiae
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Characterization of Afb, a novel bifunctional protein in Streptococcus agalactiae

机译：无乳链球菌中新型双功能蛋白Afb的表征

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Background and Objectives: Streptococcus agalactiae is the leading cause of bacterial sepsis and meningitis in newborns and results in pneumonia and bacteremia in adults. A number of S. agalactiae components are involved in colonization of target cells. Destruction of peptidoglycan and division of covalently linked daughter cells is mediated by autolysins. In this study, autolytic activity and plasma binding ability of AFb novel recombinant protein of S. agalactiae was investigated.Materials and Methods: The gbs1805 gene was cloned and expressed. E. coli strains DH5α and BL21 were used as cloning and expression hosts, respectively. After purification, antigenicity and binding ability to plasma proteins of the recombinant protein was evaluated.Results: AFb, the 18KDa protein was purified successfully. The insoluble mature protein revealed the ability to bind to fibrinogen and fibronectin. This insoluble mature protein revealed that it has the ability to bind to fibrinogen and fibronectin plasma proteins. Furthermore, in silico analysis demonstrated the AFb has an autolytic activity.Conclusions: AFb is a novel protein capable of binding to fibrinogen and fibronectin. This findings lay a ground work for further investigation of the role of the bacteria in adhesion and colonization to the host.
机译：背景与目的：无乳链球菌是新生儿细菌性败血症和脑膜炎的主要原因，并导致成人的肺炎和菌血症。许多无乳链球菌组分参与靶细胞的定殖。自溶素介导肽聚糖的破坏和共价连接的子细胞的分裂。本研究对无乳链球菌AFb新型重组蛋白的自溶活性和血浆结合能力进行了研究。材料与方法：克隆并表达了gbs1805基因。大肠杆菌菌株DH5α和BL21分别用作克隆和表达宿主。纯化后，对重组蛋白的抗原性和与血浆蛋白的结合能力进行了评价。结果：AFb成功纯化出18KDa蛋白。不溶性成熟蛋白显示出结合纤维蛋白原和纤连蛋白的能力。这种不溶的成熟蛋白表明它具有与纤维蛋白原和纤连蛋白血浆蛋白结合的能力。此外，计算机分析表明AFb具有自溶活性。结论：AFb是一种能够与纤维蛋白原和纤连蛋白结合的新型蛋白质。这一发现为进一步研究细菌在粘附和定殖到宿主中的作用奠定了基础。

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