• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Eastern Journal of Medicine >The use of methylene blue as mouthwash in periodontology
【24h】

The use of methylene blue as mouthwash in periodontology

机译：在牙周病学中使用亚甲蓝漱口水

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The use of methylene blue as mouthwash in periodontology Burak Ak 1 , Esma ?zero?lu 2 , Mehmet Taspinar 3 1 2 3 The aim of this study is to investigate the cytotoxicity effects of methylene blue on the human gingival fibroblasts cell-lines in vitro. 3T3 human gingival fibroblast cell-lines were used to evaluate the cytotoxicity of mouthwashes containing methylene blue and chlorhexidine gluconate. The cultured fibroblasts were divided into two groups which subjected into chlorhexidine gluconate or methylene blue in vitro. The cells viability was determined at 30 seconds, 1 minute and 3 minutes of exposure to mouthwashes using by XTT colorimetric assay. Spectrophotometric absorbance was measured at 550 nm using ELISA analyzer. The IC50 values were calculated for each time points for methylene blue and chlorhexidine gluconate mouthwashes. The effect of the methylene blue and chlorhexidine gluconate on the human gingival fibroblast viability was expressed as a percentage of the control groups. Comparison between two groups in different time and concentration values showed that, chlorhexidine gluconate were found to be more cytotoxic on gingival fibroblast than methylene blue. Cell viability exposured to methylene blue in 1% concentration during 3 minutes was 99% and in 100% concentration the cell viability was 88%. The chlorhexidine gluconate at the same conditions was 92% and 18% respectively. Our study demonstrates that 100% chlorhexidine gluconate (0.2% chlorhexidine gluconate concentration in commercially available products) has much more cytotoxic effect than methylene blue to human gingival fibroblast at clinical use time and different concentrations in vitro.
机译：在牙周病学中使用亚甲蓝漱口水Burak Ak 1 ，Esma？zero？lu 2 ，Mehmet Taspinar 3 1 2 3 这项研究的目的是研究亚甲基蓝对体外人牙龈成纤维细胞系的细胞毒性作用。 3T3人牙龈成纤维细胞系用于评估含亚甲基蓝和葡萄糖酸洗必泰的漱口液的细胞毒性。将培养的成纤维细胞分成两组，在体外进行葡萄糖酸氯己定或亚甲基蓝的处理。使用XTT比色测定法在暴露于漱口液的30秒，1分钟和3分钟时测定细胞活力。使用ELISA分析仪在550nm处测量分光光度吸光度。计算亚甲基蓝和葡萄糖酸洗必太漱口水每个时间点的IC50值。亚甲基蓝和葡萄糖酸氯己定对人牙龈成纤维细胞活力的影响表示为对照组的百分比。两组在不同时间和浓度值上的比较表明，葡萄糖酸氯己定对牙龈成纤维细胞的细胞毒性比亚甲基蓝更强。在3分钟内暴露于1％浓度的亚甲基蓝的细胞活力为99％，而在100％浓度下，细胞活力为88％。在相同条件下的葡萄糖酸氯己定分别为92％和18％。我们的研究表明，在临床使用时和体外不同浓度下，100％葡萄糖酸洗必太（市售产品中浓度为0.2％葡萄糖酸洗必泰）比亚甲基蓝对人齿龈成纤维细胞的细胞毒作用要强得多。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号