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The Evaluation of ANA and dsDNA results in patient with suspected autoimmune disease

机译：怀疑自身免疫性疾病患者的ANA和dsDNA结果评估

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The Evaluation of ANA and dsDNA results in patient with suspected autoimmune disease Engin Karake?e 1 , ?hsan Hakk? ?iftci 2 , Ali R?za Atasoy 2 1 2 Antinuclear antibodies (ANA) and double-stranded DNA (dsDNA) antibodies are widely used for diagnosis of autoimmune disease. The aim of this study was to investigate the prevalence of ANA and anti-dsDNA in patient with suspected autoimmune disease. Serum samples were obtained from different clinics of Sakarya Educational and Research Hospital. Each of these serum samples was tested for the presence of ANA and anti-dsDNA by ELISA technique. These tests were performed by commercial kits according to the manufacturer's instructions. ANA and anti-dsDNA results were classified as positive or negative for each patient. Borderline results were arbitrarily classified as positive. In this study, ANA and anti-dsDNA were found positive 2.96% (58/1975) and 4.52% (29/642) respectively. There is no statistical significant correlation between ANA and anti-dsDNA positivity. Only two patient both ANA and anti-dsDNA were found positivity by ELISA kits. Agreement between assays is generally marginal. ELISA technique seems to be less sensitive than fluorescent tests for ANA with fewest positivity rates. These results may be due to a number of factors which may contribute to the variability on ELISA. Finally, each of the autoantibody assays provides different criteria for diagnosis, but ANA screen test should be followed up with fluorescent tests to provide proper diagnostic information.
机译：怀疑自身免疫性疾病Engin Karake？e 1 ，“ hsan Hakk”的ANA和dsDNA结果的评估？iftci 2 ，Ali R？za Atasoy 2 1 2 抗核抗体（ANA）和双链DNA （dsDNA）抗体广泛用于自身免疫性疾病的诊断。这项研究的目的是调查可疑自身免疫性疾病患者中ANA和抗dsDNA的患病率。从萨卡里亚教育研究医院的不同诊所获得血清样品。通过ELISA技术测试这些血清样品中的每一种的ANA和抗dsDNA的存在。这些测试是根据制造商的说明通过商用套件进行的。每位患者的ANA和抗dsDNA结果分为阳性或阴性。临界结果被任意归类为阳性。在这项研究中，ANA和抗dsDNA的阳性率分别为2.96％（58/1975）和4.52％（29/642）。 ANA与抗dsDNA阳性之间没有统计学上的显着相关性。 ELISA试剂盒仅发现ANA和抗dsDNA的两名患者为阳性。测定之间的一致性通常是微不足道的。对于最低阳性率的ANA，ELISA技术似乎不如荧光测试灵敏。这些结果可能是由于许多因素可能导致ELISA的变异性。最后，每种自身抗体测定法均提供了不同的诊断标准，但是ANA筛查试验应随后进行荧光试验以提供适当的诊断信息。

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