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首页> 外文期刊>Iranian Journal of Microbiology >Comparison of four different culture media for growth of Mycobacterium avium subsp. avium isolated from naturally infected lofts of domestic pigeons.
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Comparison of four different culture media for growth of Mycobacterium avium subsp. avium isolated from naturally infected lofts of domestic pigeons.

机译：四种不同培养基对鸟分枝杆菌亚种生长的比较。从家鸽的自然感染鸽舍中分离出的鸟。

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Background and Objectives: Diagnosis of avian tuberculosis by conventional culture method is still considered as the “gold standard” technique. The main objective of this study was to compare growth of Mycobacterium avium subsp. avium on four specific Mycobacterial cultures such as glycerinated Lowenstein-Jensen medium, pyruvate-enriched Lowenstein- Jensen medium, mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium.Materials and Methods: Eighty out of more than 600 pigeons were selected based on their clinical signs and poor health conditions. The birds were numbered and their clinical signs were registered in the working sheets, and under standard condi- tion, euthanized, subjecting to necropsy examinations, followed by bacterial culture on four specific media for Mycobacterium avium subsp. avium, including glycerinated Lowenstein-Jensen (LJG) medium, pyruvate-enriched Lowenstein-Jensen medium (LJP), mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium.Results: Fifty one Mycobacterium avium subsp. avium were isolated from pigeons. Mycobactin J-supplemented Herrold-egg yolk media yielded greater number of colonies in shorter incubation time in compare with other media.Conclusion: It was concluded that most of the isolates need mycobactin as a growth factor.
机译：背景与目的：通过常规培养方法诊断禽结核仍被认为是“金标准”技术。这项研究的主要目的是比较鸟分枝杆菌亚种的生长。四种特定分枝杆菌培养物上的鸟卵，如甘油化的Lowenstein-Jensen培养基，富含丙酮酸的Lowenstein-Jensen培养基，分枝杆菌素J补充的Herrold-蛋黄蛋黄培养基和普通的Herrold-蛋黄蛋黄培养基。材料和方法：600多个鸽子中的80羽根据其临床体征和健康状况不佳进行选择。对禽进行编号，并将其临床体征记录在工作表中，并在标准条件下进行安乐死，进行尸检，然后在四种特定培养基上对鸟分枝杆菌亚种进行细菌培养。禽类，包括甘油化的Lowenstein-Jensen（LJG）培养基，富含丙酮酸的Lowenstein-Jensen培养基（LJP），分枝杆菌素J补充的Herrold-egg蛋黄培养基和普通的Herrold-egg蛋黄培养基。结果：禽分枝杆菌有51个亚种。鸟是从鸽子中分离出来的。与其他培养基相比，添加了Mycobactin J的Herrold-egg蛋黄培养基在较短的培养时间内产生了更多的菌落。结论：得出的结论是，大多数分离株都需要Mycobactin作为生长因子。

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