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首页> 外文期刊>Iranian Journal of Microbiology >Identification of Chlorophyceae based on 18S rDNA sequences from Persian Gulf.
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Identification of Chlorophyceae based on 18S rDNA sequences from Persian Gulf.

机译：根据波斯湾的18S rDNA序列鉴定绿藻科。

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Background and Objectives: Chlorophyceae are important constituents of marine phytoplankton. The taxonomy of Chlorophyceae was traditionally based solely on morphological characteristics. In the present research project, genetic diversity was investigated to analyze five species of Chlorophyceae from waters of the Persian Gulf.Materials and Methods: A clone library of the ribosomal small subunit RNA gene (18S rDNA) in the nuclear genome was constructed by PCR, and then, after examining the clones, selected clones were sequenced. The determined clone sequences were analyzed by a similarity search of the NCBI GenBank database using BLAST.Results and Conclusion: Eleven sequences were identified correctly and used for phylogenetic analysis. We identified species of Chlorophyta (Chlorella sorokiniana, Chlamydomonas sp., Neochloris aquatic, Picochlorum sp. and Nannochloris atomus) without the need to conduct extensive colony isolation techniques. Therefore, this improved molecular method can be used to generate a robust database describing the species diversity of environmental samples.
机译：背景与目的：藻科是海洋浮游植物的重要组成部分。绿藻科的分类学传统上仅基于形态特征。在本研究项目中，对遗传多样性进行了分析，以分析波斯湾水域的五种藻科。材料与方法：通过PCR构建核基因组中核糖体小亚基RNA基因（18S rDNA）的克隆文库，然后，在检查克隆后，对选定的克隆进行测序。通过使用BLAST通过NCBI GenBank数据库的相似性搜索对确定的克隆序列进行分析。结果与结论：正确鉴定了11个序列，并将其用于系统发育分析。我们无需进行广泛的菌落分离技术即可鉴定出绿藻（Chlorella sorokiniana，衣藻，衣藻，新绿藻，Picochlorum sp。和Nannochlorisatomus）的物种。因此，这种改进的分子方法可用于生成描述环境样品物种多样性的强大数据库。

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